ArticleFrontiers in cellular and infection microbiology2020
Proof of Concept of Culturomics Use of Time of Care.
Article in Frontiers in cellular and infection microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Assessing endometrial microbiota in endometriosis: culturomics and sequencing analysis of receptive-phase tissue.Current research in microbial sciences · 2026Article
- Novel gut bacteria species Paenibacillus ilasis with phosphorus degrading and soluble starch hydrolysis abilities isolated from fresh feces of rhinoceros.Scientific reports · 2025Article
- Culturomics from field-grown crop plants using dilution to extinction, two-step library preparation and amplicon sequencing.Microbiology (Reading, England) · 2025Article
- Profiling the human luminal small intestinal microbiome using a novel ingestible medical device.medRxiv : the preprint server for health sciences · 2025Article
- Impact of the ONCOBIOME network in cancer microbiome research.Nature medicine · 2025Review
- Integrative analysis of fungal communities in three types of Baijiu Daqu using third-generation sequencing and culturomics.Frontiers in microbiology · 2025Article
- Culture-based characterization of gut microbiota in inflammatory bowel disease.Frontiers in microbiology · 2025Article
- A streamlined culturomics case study for the human gut microbiota research.Scientific reports · 2024Article
- Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery.Journal of microbiology and biotechnology · 2024Review
- Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca.Antonie van Leeuwenhoek · 2024Article
- Mycobacterium ulcerans-Bordetella trematum chronic tropical cutaneous ulcer: A four-case series, Côte d'Ivoire.PLoS neglected tropical diseases · 2023Article
- Ethanol-ProducingMicroorganisms · 2023Article
- Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.Microorganisms · 2023Review
- Unveiling the Human Gastrointestinal Tract Microbiome: The Past, Present, and Future of Metagenomics.Biomedicines · 2023Review
- Comparative study of multiple approaches for identifying cultivable microalgae population diversity from freshwater samples.PloS one · 2023Article
- SVep1, a temperate phage of human oral commensalFrontiers in microbiology · 2023Article
- Characterization of metal(loid)s and antibiotic resistance in bacteria of human gut microbiota from chronic kidney disease subjects.Biological research · 2022Article
- New research frontiers pertaining to the infant gut microbiota.Microbiome research reports · 2022Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culturomics, a high throughput culture method with rapid identification of the colonies by Matrix Assisted Laser Desorption Ionization/Time Of Flight Mass Spectrometry (MALDI-TOF MS), has demonstrated its contribution to the exploration of the gut microbiota over the past 10 years. However, the cost, work time and workload, considerably limit its use on a large scale or emergency context. Here, by testing two different stool samples, including a stool sample from a patient requiring rapid immunotherapy treatment, we tested a new fast culturomic protocol using two pre-incubation media, blood culture bottle and YCFA modified medium. Both media were supplemented with 2 ml of rumen fluid filtered at 0.2 μm and 2 ml of defibrinated and sterile sheep blood. Unlike the standard culturomics, subculturing of blood culture bottle were performed at reduced incubation time (3 h, 6 h, 9 h, 24 h) and at a longer incubation time (3 days, 7 days, and 10 days) at 37°C. By testing 5,200 colonies per MALDI-TOF MS and obtaining a comparable number of cultured bacterial species (131 to 143) in a stool sample, this new protocol reduced the number of colonies tested by 57%, working time by 78.6% and cost by 72.2%. In addition, we highlighted that the proportion of strict anaerobic species has increased by 24%, known to be the preferential targets for biotherapy, including
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