ArticleFrontiers in immunology2020
Optimized Protocol for Characterization of Mouse Gut Innate Lymphoid Cells.
Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Unveiling the sensing potential of innate lymphoid cells.Communications biology · 2026Review
- Urolithin A activates aryl hydrocarbon receptor-NLRP6-mediated pathways in intestinal epithelial cells to modulate mucosal immunity and strengthen gut barrier integrity.Nature communications · 2026Article
- CD177⁺ neutrophil-platelet aggregates contribute to thromboinflammation via NETs in necrotizing enterocolitis.Nature communications · 2026Article
- CD4 T cell therapy counteracts inflammaging and senescence by preserving gut barrier integrity.Science immunology · 2025Article
- Ex Vivo Irradiation Protocol in Murine Lymph Node Cells.Bulletin of experimental biology and medicine · 2025Article
- KLRG1 identifies regulatory T cells with mitochondrial alterations that accumulate with aging.Nature aging · 2025Article
- Annexin A1 levels affect microbiota in health and DSS-induced colitis/inflammatory bowel disease development.Frontiers in immunology · 2025Article
- Effect of acetylcholinesterase inhibition on immune cells in the murine intestinal mucosa.Heliyon · 2024Article
- Renal protective and immunoregulatory effects ofFrontiers in nutrition · 2024Article
- CD200R1 promotes interleukin-17 production by group 3 innate lymphoid cells by enhancing signal transducer and activator of transcription 3 activation.Mucosal immunology · 2023Article
- OMIP-086: Full spectrum flow cytometry for high-dimensional immunophenotyping of mouse innate lymphoid cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- ILC3: a case of conflicted identity.Frontiers in immunology · 2023Review
- Flow cytometric analysis of innate lymphoid cells: challenges and solutions.Frontiers in immunology · 2023Review
- Impact of IFN-y and CD40 signalling on Toxoplasma gondii cyst formation in differentiated Neuro-2a neuroblastoma cells.Parasite immunology · 2022Article
- Innate lymphoid cells type 3 in cancer.Frontiers in immunology · 2022Review
- Innate Lymphoid Cells in Intestinal Homeostasis and Inflammatory Bowel Disease.International journal of molecular sciences · 2021Review
- Type 3 Innate Lymphoid Cells as Regulators of the Host-Pathogen Interaction.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since their discovery, innate lymphoid cells (ILCs) have gradually been gaining greater relevance in the field of immunology due to their multiple functions in the innate immune response. They can mainly be found in mucosal and barrier organs like skin, gut, and lungs, and have been classified into five main types (NKs, ILC1s, ILC2s, ILC3s, and Lti cells) according to their function and development. They all play major roles in functions such as tissue homeostasis, early pathogen defense, regulation of inflammation, or tissue remodeling. ILCs are mostly tissue-resident cells tightly bound to the tissue structure, a fact that requires long and complex protocols that do not always provide sufficient yield for analysis. This suggests the need for optimized approaches aimed at ensuring that enriched and viable ILC samples are obtained, in order to furnish quality results. Herein a detailed protocol is established for obtaining a single-cell suspension highly enriched in lymphoid cells from mouse gut in order to identify the different subsets of ILCs by means of flow cytometry. The cell marker panel and flow cytometry gating strategies for identification and quantification of all the different ILC populations are provided for simultaneous analysis. Moreover, the protocol described includes a procedure for studying the different cytokines produced by ILC3s involved in maintaining the integrity of the gut barrier and defending against extracellular pathogens. As a result, herein an efficient method is presented for studying mouse ILCs within the lamina propria of the small intestine and colon; this can constitute a useful tool for future investigations in the field.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.