ArticleFrontiers in behavioral neuroscience2020
Chemogenetic Stimulation and Silencing of the Insula, Amygdala, Nucleus Accumbens, and Their Connections Differentially Modulate Alcohol Drinking in Rats.
Article in Frontiers in behavioral neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Sex differences in insular cortex function in persistent alcohol drinking despite aversion in mice.Molecular psychiatry · 2026Article
- INTERFERON-REGULATORY FACTOR 7: A NEUROIMMUNE ROLE FOR VAPOR-INDUCED ESCALATIONS IN ETHANOL SELF-ADMINISTRATION.bioRxiv : the preprint server for biology · 2026Article
- Anterior Insula Activity during Alcohol and Social Reward Self-Administration and Choice in Male and Female Rats.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Advances in chemogenetics: a review of DREADDs and its application in psychiatric disorders.Molecular psychiatry · 2026Review
- Nucleus accumbens core chemogenetic excitation in male mice and chemogenetic inhibition in female mice reduced ethanol reward.Biology of sex differences · 2025Article
- Repeated cycles of binge-like ethanol consumption and abstinence alter neuropeptide mRNA in prefrontal and insular cortex, amygdala, and lateral hypothalamus of male and female C57BL/6J mice.Alcohol, clinical & experimental research · 2025Article
- Insular cortex subregions have distinct roles in cued heroin seeking after extinction learning and prolonged withdrawal in rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Converging Effects of Chronic Pain and Binge Alcohol Consumption on Anterior Insular Cortex Neurons Projecting to the Dorsolateral Striatum in Male Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Still a "hidden island"? The rodent insular cortex in drug seeking, reward, and risk.Neuroscience and biobehavioral reviews · 2023Review
- Recruitment of Hippocampal and Thalamic Pathways to the Central Amygdala in the Control of Feeding Behavior Under Novelty.Research square · 2023Article
- Using lickometry to infer differential contributions of salience network regions during compulsion-like alcohol drinking.Addiction neuroscience · 2023Article
- Selective inhibition of excitatory synaptic transmission alters the emergent bursting dynamics ofFrontiers in neural circuits · 2023Article
- Activation of the dorsal septum increases alcohol consumption in male C57BL/6J mice.Addiction neuroscience · 2022Article
- The Toll-like receptor 7 agonist imiquimod increases ethanol self-administration and induces expression of Toll-like receptor related genes.Addiction biology · 2022Article
- Astrocytes play a critical role in mediating the effect of acute ethanol on central amygdala glutamatergic transmission.Neuropharmacology · 2022Article
- Article
- Adaptation of the 5-choice serial reaction time task to measure engagement and motivation for alcohol in mice.Frontiers in behavioral neuroscience · 2022Article
- Subregional Differences in Alcohol Modulation of Central Amygdala Neurocircuitry.Frontiers in molecular neuroscience · 2022Review
- Better living through understanding the insula: Why subregions can make all the difference.Neuropharmacology · 2021Review
- The role of anterior insula-brainstem projections and alpha-1 noradrenergic receptors for compulsion-like and alcohol-only drinking.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anterior insular cortex is hypothesized to represent interoceptive effects of drug reward in the service of goal-directed behavior. The insula is richly connected, but the insula circuitry in addiction remains poorly characterized. We examined the involvement of the anterior insula, amygdala, and nucleus accumbens, as well as the projections of the anterior insula to the central amygdala, basolateral amygdala (BLA), and nucleus accumbens core in voluntary alcohol drinking. We trained alcohol-preferring Alko Alcohol (AA) rats to drink alcohol during intermittent 2-h sessions. We then expressed excitatory or inhibitory designer receptors [designer receptors exclusively activated by designer drugs (DREADDs)] in the anterior insula, nucleus accumbens, or amygdala by means of adenovirus-mediated gene transfer and activated the DREADDs with clozapine-N-oxide (CNO) prior to the drinking sessions. Next, to examine the role of specific insula projections, we expressed FLEX-DREADDs in the efferent insula → nucleus accumbens core, insula → central amygdala, and insula → BLA projections by means of a retrograde AAV-Cre vector injected into the insula projection areas. In the anterior insula and amygdala, excitatory Gq-DREADDs significantly attenuated alcohol consumption. In contrast, in the nucleus accumbens, the Gq-DREADD stimulation increased alcohol drinking, and the inhibitory Gi-DREADDs suppressed it. The Gq-DREADDs expressed in the insula → nucleus accumbens core and insula → central amygdala projections increased alcohol intake, whereas inhibition of these projections had no effect. These data demonstrate that the anterior insula, along with the amygdala and nucleus accumbens, has a key role in controlling alcohol drinking by providing excitatory input to the central amygdala and nucleus accumbens to enhance alcohol reward.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.