ArticleFrontiers in neuroanatomy2020
Comparison of Transparency and Shrinkage During Clearing of Insect Brains Using Media With Tunable Refractive Index.
Article in Frontiers in neuroanatomy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies.Scientific reports · 2025Article
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- Fluid preservation in brain banking: a review.Free neuropathology · 2024Article
- Dynamic interaction of injected liquid jet with skin layer interfaces revealed by microsecond imaging of optically cleared ex vivo skin tissue model.Journal of biological engineering · 2023Article
- Microglial morphometric analysis: so many options, so little consistency.Frontiers in neuroinformatics · 2023Review
- Review
- Antibody-Based Methods Reveal the Protein Expression Properties of Glucosinolate Sulfatase 1 and 2 in Plutella xylostella.Journal of insect science (Online) · 2022Article
- Long Preservation of AAV-Transduced Fluorescence by a Modified Organic Solvent-Based Clearing Method.International journal of molecular sciences · 2022Article
- GFAP splice variants fine-tune glioma cell invasion and tumour dynamics by modulating migration persistence.Scientific reports · 2022Article
- A new, fluorescence-based method for visualizing the pseudopupil and assessing optical acuity in the dark compound eyes of honeybees and other insects.Scientific reports · 2021Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- Modeling Nonlinear Dendritic Processing of Facilitation in a Dragonfly Target-Tracking Neuron.Frontiers in neural circuits · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improvement of imaging quality has the potential to visualize previously unseen building blocks of the brain and is therefore one of the great challenges in neuroscience. Rapid development of new tissue clearing techniques in recent years have attempted to solve imaging compromises in thick brain samples, particularly for high resolution optical microscopy, where the clearing medium needs to match the high refractive index of the objective immersion medium. These problems are exacerbated in insect tissue, where numerous (initially air-filled) tracheal tubes branching throughout the brain increase the scattering of light. To date, surprisingly few studies have systematically quantified the benefits of such clearing methods using objective transparency and tissue shrinkage measurements. In this study we compare a traditional and widely used insect clearing medium, methyl salicylate combined with permanent mounting in Permount ("MS/P") with several more recently applied clearing media that offer tunable refractive index (
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