ArticleScientific reports2020
An advanced genetic toolkit for exploring the biology of the rock-inhabiting black fungus Knufia petricola.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Carbon and nitrogen availability affects biofilm growth and morphology of the extremotolerant fungusApplied and environmental microbiology · 2026Article
- Black yeasts are efficient heterologous hosts for the production of a wide range of fungal polyketides.Nature chemical biology · 2026Article
- Iron acquisition strategies of the rock-inhabiting fungusApplied and environmental microbiology · 2026Article
- Genomic Insights Into Convergent Evolution: Adaptation to Rocky Habitats in Rock-Inhabiting Fungi.Molecular biology and evolution · 2025Article
- Conventional and innovative approaches to black fungi control for stone heritage preservation.IUBMB life · 2025Review
- 1,8-Dihydroxynaphthalene (DHN) melanin provides unequal protection to black fungi Knufia petricola and Cryomyces antarcticus from UV-B radiation.Environmental microbiology reports · 2024Article
- Deletion of the polyketide synthase-encoding gene pks1 prevents melanization in the extremophilic fungus Cryomyces antarcticus.IUBMB life · 2024Article
- A single septin from a polyextremotolerant yeast recapitulates many canonical functions of septin hetero-oligomers.Molecular biology of the cell · 2024Article
- The Tet-on system for controllable gene expression in the rock-inhabiting black fungus Knufia petricola.Extremophiles : life under extreme conditions · 2024Article
- Expanding the Toolbox for Genetic Manipulation inJournal of fungi (Basel, Switzerland) · 2024Article
- Genetic Engineering of the Rock InhabitantFrontiers in fungal biology · 2022Article
- CRISPR/Cas9 RNP-assisted validation of palmarumycin biosynthetic gene cluster inFrontiers in microbiology · 2022Article
- Review
- Variations in the Community Structure of Fungal Microbiota Associated with Apple Fruit Shaped by Fruit Bagging-Based Practice.Journal of fungi (Basel, Switzerland) · 2021Article
- Effects of Simulated Microgravity on the Proteome and Secretome of the Polyextremotolerant Black FungusFrontiers in genetics · 2021Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Microcolonial black fungi are a group of ascomycetes that exhibit high stress tolerance, yeast-like growth and constitutive melanin formation. They dominate a range of hostile natural and man-made environments, from desert rocks and salterns to dishwashers, roofs and solar panels. Due to their slow growth and a lack of genetic tools, the underlying mechanisms of black fungi's phenotypic traits have remained largely unexplored. We chose to address this gap by genetically engineering the rock-inhabiting fungus Knufia petricola (Eurotiomycetes, Chaetothyriales), a species that exhibits all characteristics of black fungi. A cell biological approach was taken by generating K. petricola strains expressing green or red fluorescent protein variants. By applying: (1) traditional gene replacement; (2) gene editing and replacement via plasmid-based or ribonucleoprotein (RNP)-based CRISPR/Cas9, and (3) silencing by RNA interference (RNAi), we constructed mutants in the pathways leading to melanin, carotenoids, uracil and adenine. Stable single and double mutants were generated with homologous recombination (HR) rates up to 100%. Efficient, partially cloning-free strategies to mutate multiple genes with or without resistance cassettes were developed. This state-of-the-art genetic toolkit, together with the annotated genome sequence of strain A95, firmly established K. petricola as a model for exploring microcolonial black fungi.
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