ArticleScientific reports2020
A rapid colorimetric LAMP assay for detection of Rhizoctonia solani AG-1 IA causing sheath blight of rice.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 34 citations in OpenAlex.
- Development of a multipurpose diagnostic marker using PCR, real-time PCR, and LAMP assays for rapid detection ofFrontiers in plant science · 2026Article
- Cinchona-based liquid formulation exhibits antifungal activity through Tryptophan starvation and disruption of mitochondrial respiration in Rhizoctonia Solani.Scientific reports · 2025Article
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- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
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- Active vs. Passive Thermal Imaging for Helping the Early Detection of Soil-Borne Rot Diseases on Wild Rocket [Plants (Basel, Switzerland) · 2023Article
- Molecular diagnostic assay for pre-harvest detection ofFrontiers in microbiology · 2023Article
- Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes.Communications biology · 2022Article
- Rapid and Accurate Detection ofPathogens (Basel, Switzerland) · 2022Article
- Gene expression analysis of resistant and susceptible rice cultivars to sheath blight after inoculation with Rhizoctonia solani.BMC genomics · 2022Article
- Global distribution, traditional and modern detection, diagnostic, and management approaches ofFrontiers in microbiology · 2022Review
- Colorimetric loop-mediated isothermal amplification assay for detection and ecological monitoring ofFrontiers in plant science · 2022Article
- DNA barcoding of phytopathogens for disease diagnostics and bio-surveillance.World journal of microbiology & biotechnology · 2021Review
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Authors and funding
9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Rhizoctonia solani is one of the most devastating pathogens. R. solani AG-1 IA causes sheath blight in rice, maize, and other Gramineous plants. Accurate identification is essential for the effective management of this pathogen. In the present study, a set of four primers were designed viz. RSPG1, RSPG2, RSPG4, and RSPG5 for polygalacturonase (PG) gene, an important virulence factor in phytopathogenic fungi. All four primer sets showed specific amplification of 300 bp (RSPG1F/R), 375 bp (RSPG2F/R), 500 bp (RSPG4F/R) and 336 bp (RSPG5F/R) amplicons. q-PCR detection using each primer sets could detect up to 10 pg of DNA. We also designed six primers (RS_pg_F3_1/RS_pg_B3_1, RS_pg_FIP_1.1/RS-pg_BIP_1.1, and RS_pg_LF_1/RS_pg_LB_1) for PG gene. Further, a colorimetric LAMP assay developed yielded visual confirmation of the pathogen within 45 min of sample collection when coupled with rapid high throughput template preparation method (rHTTP) from infected samples. The sensitivity of the LAMP assay was as low as 1.65 fg/µl of template DNA and could effectively detect R. solani AG-1 IA from diseased plant tissues and soil samples. The LAMP assay was highly specific for R. solani as it did not show any amplification with other AG groups of R. solani and closely related fungal and bacterial outgroups. This study will help in designing an effective point of care diagnostic method for early monitoring of R. solani and thereby planning timely preventive measures against the pathogen.
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