ArticleHorticulture research2020
Brassica rapa orphan genes largely affect soluble sugar metabolism.
Article in Horticulture research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Metabolomics and Transcriptomics Reveal the Function of Trigonelline and Its Synthesis Gene BrNANMT in Clubroot Susceptibility of Brassica rapa.Plant, cell & environment · 2026Article
- Construction of an Overexpression Library for Chinese Cabbage Orphan Genes inPlants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of Orphan Genes Within Coriander.Plants (Basel, Switzerland) · 2025Article
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- Construction and evaluation ofFrontiers in plant science · 2025Article
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Orphan geneHorticulture research · 2024Article
- Chinese cabbage orphan geneFrontiers in plant science · 2024Article
- Role of Brassica orphan gene BrLFM on leafy head formation in Chinese cabbage (Brassica rapa).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- The Lost and Found: Unraveling the Functions of Orphan Genes.Journal of developmental biology · 2023Review
- LED omics in Rocket Salad (Plants (Basel, Switzerland) · 2023Article
- Article
- Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.Frontiers in plant science · 2023Article
- Orphan Genes in Crop Improvement: Enhancing Potato Tuber Protein without Impacting Yield.Plants (Basel, Switzerland) · 2022Article
- Comparative Metabolic Study of Two Contrasting Chinese Cabbage Genotypes under Mild and Severe Drought Stress.International journal of molecular sciences · 2022Article
- Genome Editing for Improving Crop Nutrition.Frontiers in genome editing · 2022Review
- CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security.Frontiers in genetics · 2022Review
- Research Advances and Prospects of Orphan Genes in Plants.Frontiers in plant science · 2022Review
- Taxonomically Restricted Genes Are Associated With Responses to Biotic and Abiotic Stresses in Sugarcane (Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Orphan genes (OGs), which are genes unique to a specific taxon, play a vital role in primary metabolism. However, little is known about the functional significance of Brassica rapa OGs (BrOGs) that were identified in our previous study. To study their biological functions, we developed a BrOG overexpression (BrOGOE) mutant library of 43 genes in Arabidopsis thaliana and assessed the phenotypic variation of the plants. We found that 19 of the 43 BrOGOE mutants displayed a mutant phenotype and 42 showed a variable soluble sugar content. One mutant, BrOG1OE, with significantly elevated fructose, glucose, and total sugar contents but a reduced sucrose content, was selected for in-depth analysis. BrOG1OE showed reduced expression and activity of the Arabidopsis sucrose synthase gene (AtSUS); however, the activity of invertase was unchanged. In contrast, silencing of two copies of BrOG1 in B. rapa, BraA08002322 (BrOG1A) and BraSca000221 (BrOG1B), by the use of an efficient CRISPR/Cas9 system of Chinese cabbage (B. rapa ssp. campestris) resulted in decreased fructose, glucose, and total soluble sugar contents because of the upregulation of BrSUS1b, BrSUS3, and, specifically, the BrSUS5 gene in the edited BrOG1 transgenic line. In addition, we observed increased sucrose content and SUS activity in the BrOG1 mutants, with the activity of invertase remaining unchanged. Thus, BrOG1 probably affected soluble sugar metabolism in a SUS-dependent manner. This is the first report investigating the function of BrOGs with respect to soluble sugar metabolism and reinforced the idea that OGs are a valuable resource for nutrient metabolism.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.