ArticleHorticulture research2020
Clay nanosheet-mediated delivery of recombinant plasmids expressing artificial miRNAs via leaf spray to prevent infection by plant DNA viruses.
Article in Horticulture research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 65 citations in OpenAlex.
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Molecular Targeted Suppression of Male Fertility in Amaranthus palmeri S. Watson: Function and Layered Double Hydroxide Nanosheets-Based Delivery System of ApmiR319.Plant biotechnology journal · 2026Article
- Genetic Transformation of Catharanthus roseus with Simplified Nanocarrier-Based Gene Delivery Method Using Green Synthesized Superparamagnetic Iron Oxide Nanoparticles.Molecular biotechnology · 2026Article
- amiR414e-mediated silencing confers resistance to Cotton leaf curl Multan virus in cotton.Molecular biology reports · 2026Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Modified carbon dot-mediated transient transformation for genomic and epigenomic studies in wheat.Plant biotechnology journal · 2025Article
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Nanodelivery of nucleic acids for plant genetic engineering.Discover nano · 2025Review
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Form and Function: The Factors That Influence the Efficacy of Nanomaterials for Gene Transfer to Plants.Molecules (Basel, Switzerland) · 2025Review
- Biochemical and nanotechnological approaches to combat phytoparasitic nematodes.Plant biotechnology journal · 2024Review
- Enhancing iron content and growth of cucumber seedlings with MgFe-LDHs under low-temperature stress.Journal of nanobiotechnology · 2024Article
- Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023Review
- Enhancing lettuce yield via Cu/Fe-layered double hydroxide nanoparticles spraying.Journal of nanobiotechnology · 2023Article
- RNA Interference Past and Future Applications in Plants.International journal of molecular sciences · 2023Review
- Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Frontiers in plant science · 2023Review
- The Promising Nanovectors for Gene Delivery in Plant Genome Engineering.International journal of molecular sciences · 2022Review
- Clays as Vehicles for Drug Photostability.Pharmaceutics · 2022Review
- Nanomaterials coupled with microRNAs for alleviating plant stress: a new opening towards sustainable agriculture.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 2 countries.
Funding
Abstract
Whitefly-transmitted begomoviruses are economically important plant pathogens that cause severe problems in many crop plants, such as tomato, papaya, cotton, and tobacco. Tomato yellow leaf curl virus (TYLCV) is a typical monopartite begomovirus that has been extensively studied, but methods that can efficiently control begomoviruses are still scarce. In this study, we combined artificial microRNA (amiRNA)-mediated silencing technology and clay nanosheet-mediated delivery by spraying and developed a method for efficiently preventing TYLCV infection in tomato plants. We designed three amiRNAs that target different regions of TYLCV to silence virus-produced transcripts. Three plant expression vectors expressing pre-amiRNAs were constructed, and recombinant plasmid DNAs (pDNAs) were loaded onto nontoxic and degradable layered double hydroxide (LDH) clay nanosheets. LDH nanosheets containing multiple pDNAs were sprayed onto plant leaves. We found that the designed amiRNAs were significantly accumulated in leaves 7 days after spraying, while the pDNAs were sustainably detected for 35 days after the spray, suggesting that the LDH nanosheets released pDNAs in a sustained manner, protected pDNAs from degradation and efficiently delivered pDNAs into plant cells. Importantly, when the LDH nanosheets coated with pDNAs were sprayed onto plants infected by TYLCV, both the disease severity and TYLCV viral concentration in sprayed plants were significantly decreased during the 35 days, while the levels of H
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.