ArticleHaematologica2022
Post-translational polymodification of β1-tubulin regulates motor protein localisation in platelet production and function.
Article in Haematologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Sulfur valence normalization to expand quantitative peptide selection in protein metrology.Analytical and bioanalytical chemistry · 2026Article
- Glucagon secretion by pancreatic alpha-cells requires an intact tubulin-cytoskeleton-primary cilium axis.Molecular medicine (Cambridge, Mass.) · 2026Article
- Engine breakdown of lysosomes and related organelles and the resulting physiology.Frontiers in cell and developmental biology · 2025Review
- Cell cycle-dependent centrosome clustering precedes proplatelet formation.Science advances · 2024Article
- Dynamic actin/septin network in megakaryocytes coordinates proplatelet elaboration.Haematologica · 2024Article
- Human Bone Marrow Organoids for Disease Modeling, Discovery, and Validation of Therapeutic Targets in Hematologic Malignancies.Cancer discovery · 2023Article
- Preferential uptake of SARS-CoV-2 by pericytes potentiates vascular damage and permeability in an organoid model of the microvasculature.Cardiovascular research · 2022Article
- Impaired microtubule dynamics contribute to microthrombocytopenia in RhoB-deficient mice.Blood advances · 2022Article
- Sorting nexin 24 is required for α-granule biogenesis and cargo delivery in megakaryocytes.Haematologica · 2022Article
- Cucurbitacins Elicit Anti-Platelet Activity via Perturbation of the Cytoskeleton and Integrin Function.Thrombosis and haemostasis · 2022Article
- Don't you forget about me(gakaryocytes).Blood · 2022Article
- Article
- The triple crown of platelet generation.Blood · 2022Article
- Article
- Novel gene variants in patients with platelet-based bleeding using combined exome sequencing and RNAseq murine expression data.Journal of thrombosis and haemostasis : JTH · 2021Article
- SMIFH2 inhibition of platelets demonstrates a critical role for formin proteins in platelet cytoskeletal dynamics.Journal of thrombosis and haemostasis : JTH · 2020Article
- Tubulin in Platelets: When the Shape Matters.International journal of molecular sciences · 2019Review
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
In specialised cells, the expression of specific tubulin isoforms and their subsequent post-translational modifications drive and coordinate unique morphologies and behaviours. The mechanisms by which β1-tubulin, the platelet and megakaryocyte (MK) lineage restricted tubulin isoform, drives platelet production and function remains poorly understood. We investigated the roles of two key post-translational tubulin polymodifications (polyglutamylation and polyglycylation) on these processes using a cohort of thrombocytopenic patients, human induced pluripotent stem cell (iPSC) derived MKs, and healthy human donor platelets. We find distinct patterns of polymodification in MKs and platelets, mediated by the antagonistic activities of the cell specific expression of Tubulin Tyrosine Ligase Like (TTLLs) and Cytosolic Carboxypeptidase (CCP) enzymes. The resulting microtubule patterning spatially regulates motor proteins to drive proplatelet formation in megakaryocytes, and the cytoskeletal reorganisation required for thrombus formation. This work is the first to show a reversible system of polymodification by which different cell specific functions are achieved.
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