ArticleVeterinary medicine and science2021
Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter.
Article in Veterinary medicine and science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 4 citations in OpenAlex.
- A Simple and Versatile Method for Ex Vivo Monitoring of Goat Vaginal Mucosa Transduction by Viral Vector Vaccines.Vaccines · 2024Article
- Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response.Frontiers in cellular and infection microbiology · 2022Article
- Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter.Veterinary medicine and science · 2021Article
- A Sequential Study on the Pathology of Peste Des Petits Ruminants and Tissue Distribution of the Virus Following Experimental Infection of Black Bengal Goats.Frontiers in veterinary science · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTraditionally isolation of peste des petits ruminant virus (PPRV) is performed in Vero cells that takes several blind passages before observing typical cytopathic effects (CPEs). As an alternate, researchers have been using lamb kidney (LK) cells but day-old lambs are difficult to obtain and requires animal sacrifice.
objectiveWe established a primary goat kidney (GK) cell culture from the kidneys obtained at slaughter.
methodsThe kidney of Black Bengal goats were collected from slaughter house and processed to make single cell suspension. The cells were resuspended in appropriate culture medium and maintained under optimum culture condition.
resultsThe 80% confluent monolayer of GK cells was obtained after 15-20 days post seeding. Upon infection with a field isolate of PPRV, the well-developed CPEs characterized by cell rounding, vacuolation in the cytoplasm and fusion of cells were observed after 48 hr post infection. Virus quantification in the culture supernatant revealed more viral RNA in GK cells than LK cells. The multicycle growth analysis of PPRV showed a steady increase in the virus loads in the culture supernatant of infected GK cells, suggesting an adaptation of the PPRV in GK cells.
conclusionsThe findings suggest that primary GK cells can be successfully prepared from the mature kidney cortical tissues and can be used for the isolation of PPRV. This system could reduce the unnecessary sacrifice of lambs or kids. Since kidneys of slaughtered goats are available throughout the year, using this protocol primary cell culture from mature goat kidney can provide primary cells to the laboratory throughout the year.
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