ArticlePLoS neglected tropical diseases2020
The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- A qPCR identification scheme to differentiate actinomycetoma from eumycetoma.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Simultaneous Detection of Four Madurella Species Using Loop-Mediated Isothermal Amplification (LAMP) for Eumycetoma Diagnosis.Mycopathologia · 2025Article
- The development of a multiplex recombinase polymerase amplification reaction to detect the most common causative agents of eumycetoma.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- A Longstanding Case of Black Grain Mycetoma in a Somalian Male Patient: A Case Report.Clinical case reports · 2025Article
- A study of case management challenge for black grain eumycetoma during the ongoing war in Sudan.Clinical case reports · 2024Article
- An updated list of eumycetoma causative agents and their differences in grain formation and treatment response.Clinical microbiology reviews · 2024Review
- The nutritional status of mycetoma affected patients seen at the Mycetoma Research Center, Sudan.PLoS neglected tropical diseases · 2024Article
- Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent.PLoS neglected tropical diseases · 2023Article
- The First Case ofJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan.PLoS neglected tropical diseases · 2022Article
- Eumycetoma with pulmonary dissemination an unusual complication: Case series and literature review.PLoS neglected tropical diseases · 2022Review
- Ultrasound-guided fine-needle aspiration cytology significantly improved mycetoma diagnosis.Journal of the European Academy of Dermatology and Venereology : JEADV · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEumycetoma is a neglected tropical disease most commonly caused by the fungus Madurella mycetomatis. Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current M. mycetomatis specific PCR primers were recently discovered to cross-react with Madurella pseudomycetomatis. Here, we used a comparative genome approach to develop a new M. mycetomatis specific PCR for species identification. METHODOLOGY: Predicted-protein coding sequences unique to M. mycetomatis were first identified in BLASTCLUST based on E-value, size and presence of orthologues. Primers were then developed for 16 unique sequences and evaluated against 60 M. mycetomatis isolates and other eumycetoma causing agents including the Madurella sibling species. Out of the 16, only one was found to be specific to M. mycetomatis.
conclusionWe have discovered a predicted-protein coding sequence unique to M. mycetomatis and have developed a new species-specific PCR to be used as a novel diagnostic marker for M. mycetomatis.
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