ArticleAnalytical chemistry2021
Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA).
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Formulation Strategies for Immunomodulatory Natural Products in 3D Tumor Spheroids and Organoids: Current Challenges and Emerging Solutions.Pharmaceutics · 2025Review
- Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.Biomicrofluidics · 2025Review
- An acoustofluidic embedding platform for rapid multiphase microparticle injection.Nature communications · 2025Article
- A Facile Surface Modification Strategy for Antibody Immobilization on 3D-Printed Surfaces.Biosensors · 2025Article
- Parallel sample processing for mass spectrometry-based single cell proteomics.Analytica chimica acta · 2024Article
- Open and closed microfluidics for biosensing.Materials today. Bio · 2024Review
- A highly sensitive point-of-care detection platform for Salmonella typhimurium by integrating magnetic enrichment and fluorescent CsPbBrMikrochimica acta · 2024Article
- Establishment of an indirect competitive immunoassay for the detection of dicamba based on a highly specific nanobody.The Science of the total environment · 2024Article
- Galvanic Displacement Reaction Enabled Specific and Sensitive Detection of Bacteria with a Digital Photocorrosion GaAs/AlGaAs Biosensor.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Article
- A 3D printed microfluidic device for scalable multiplexed CRISPR-cas12a biosensing.Biomedical microdevices · 2023Article
- 3D printed spinning cup-shaped device for immunoaffinity solid-phase extraction of diclofenac in wastewaters.Mikrochimica acta · 2022Article
- Molecular diagnostics in neurotrauma: Are there reliable biomarkers and effective methods for their detection?Frontiers in molecular biosciences · 2022Review
- Machine-Learning-Assisted Microfluidic Nanoplasmonic Digital Immunoassay for Cytokine Storm Profiling in COVID-19 Patients.ACS nano · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Enzyme-linked immunosorbent assay (ELISA) is the gold standard method for protein biomarkers. However, scaling up ELISA for multiplexed biomarker analysis is not a trivial task due to the lengthy procedures for fluid manipulation and high reagent/sample consumption. Herein, we present a highly scalable multiplexed ELISA that achieves a similar level of performance to commercial single-target ELISA kits as well as shorter assay time, less consumption, and simpler procedures. This ELISA is enabled by a novel microscale fluid manipulation method, composable microfluidic plates (cPlate), which are comprised of miniaturized 96-well plates and their corresponding channel plates. By assembling and disassembling the plates, all of the fluid manipulations for 96 independent ELISA reactions can be achieved simultaneously without any external fluid manipulation equipment. Simultaneous quantification of four protein biomarkers in serum samples is demonstrated with the cPlate system, achieving high sensitivity and specificity (∼ pg/mL), short assay time (∼1 h), low consumption (∼5 μL/well), high scalability, and ease of use. This platform is further applied to probe the levels of three protein biomarkers related to vascular dysfunction under pulmonary nanoparticle exposure in rat's plasma. Because of the low cost, portability, and instrument-free nature of the cPlate system, it will have great potential for multiplexed point-of-care testing in resource-limited regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.