ArticleThe Journal of cell biology2021
3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.
Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 112 citations in OpenAlex.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Classifying biophysical subpopulations of insulin secretory granules using quantitative whole-cell structure analysis.Structure (London, England : 1993) · 2026Article
- Axonemal Dynein Visualized in Primary Cilia via Expansion Microscopy.Cytoskeleton (Hoboken, N.J.) · 2026Article
- vEMRec: High-Resolution Volume Electron Microscopy Reconstruction Based on Structure-Preserving and High-Fidelity 3D Alignment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- vEMINR: Ultra-Fast Isotropic Reconstruction for Volume Electron Microscopy With Implicit Neural Representation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A scoping study of the whole-cell imaging literature as a foundation for the emerging field of cell anatomy.BMC biology · 2026Article
- Explainable AI-based analysis of human pancreas sections identifies traits of type 2 diabetes.Nature communications · 2026Article
- Close-up of vesicular ER exit sites by volume electron imaging using FIB-SEM.The Journal of cell biology · 2026Article
- Insights into Microtubule Nucleation at the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- Golgi-Associated Protein JAKMIP2 Is Linked to the Centrosome and Performs Microtubule-Related Functions.Cells · 2025Article
- MitoEM 2.0: A Benchmark for Challenging 3D Mitochondria Instance Segmentation from EM Images.bioRxiv : the preprint server for biology · 2025Article
- A guide to characterizing the dynamic mitochondria-endoplasmic reticulum contact sites.The FEBS journal · 2025Review
- Roles of molecular motors in insulin-secreting beta cells.Current opinion in cell biology · 2025Review
- Secretory stimuli distinctly regulate insulin secretory granule maturation through structural remodeling.Structure (London, England : 1993) · 2025Article
- Article
- Classifying Biophysical Subpopulations of Insulin Secretory Granules using Quantitative Whole Cell Structure Analysis.bioRxiv : the preprint server for biology · 2025Article
- Deep mapping of thebioRxiv : the preprint server for biology · 2025Article
- Directed insulin secretion from beta cells occurs at cortical sites devoid of microtubules at the edges of ELKS/LL5β patches.Molecular biology of the cell · 2025Article
- A scoping study of the whole-cell imaging literature: a foundational corpus, potential for mesoscale data synthesis, and implications for standardization of an emerging field.bioRxiv : the preprint server for biology · 2025Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
Abstract
Microtubules play a major role in intracellular trafficking of vesicles in endocrine cells. Detailed knowledge of microtubule organization and their relation to other cell constituents is crucial for understanding cell function. However, their role in insulin transport and secretion is under debate. Here, we use FIB-SEM to image islet β cells in their entirety with unprecedented resolution. We reconstruct mitochondria, Golgi apparati, centrioles, insulin secretory granules, and microtubules of seven β cells, and generate a comprehensive spatial map of microtubule-organelle interactions. We find that microtubules form nonradial networks that are predominantly not connected to either centrioles or endomembranes. Microtubule number and length, but not microtubule polymer density, vary with glucose stimulation. Furthermore, insulin secretory granules are enriched near the plasma membrane, where they associate with microtubules. In summary, we provide the first 3D reconstructions of complete microtubule networks in primary mammalian cells together with evidence regarding their importance for insulin secretory granule positioning and thus their supportive role in insulin secretion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.