ArticleJournal of biomedical optics2020
Diagnosing 12 prostate needle cores within an hour of biopsy via open-top light-sheet microscopy.
Article in Journal of biomedical optics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies.Scientific reports · 2025Article
- Integrating nonlinear optical and Raman spectral imaging for label-free pathological examinations.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.Molecular psychiatry · 2024Review
- Blueprints from plane to space: outlook of next-generation three-dimensional histopathology.Cancer science · 2024Review
- Optical imaging for screening and early cancer diagnosis in low-resource settings.Nature reviews bioengineering · 2024Article
- Digital pathology systems enabling quality patient care.Genes, chromosomes & cancer · 2023Review
- Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy.IEEE transactions on bio-medical engineering · 2023Article
- Rapid digital pathology of H&E-stained fresh human brain specimens as an alternative to frozen biopsy.Communications medicine · 2023Article
- Rapid On-Site Microscopy and Mapping of Diagnostic Biopsies for See-And-Treat Guidance of Localized Prostate Cancer Therapy.Cancers · 2023Article
- Rapid and label-free histological imaging of unprocessed surgical tissues via dark-field reflectance ultraviolet microscopy.iScience · 2023Article
- Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2022Article
- Label-free multimodal nonlinear optical imaging of needle biopsy cores for intraoperative cancer diagnosis.Journal of biomedical optics · 2022Article
- Slide Over: Advances in Slide-Free Optical Microscopy as Drivers of Diagnostic Pathology.The American journal of pathology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
significanceProcessing and diagnosing a set of 12 prostate biopsies using conventional histology methods typically take at least one day. A rapid and accurate process performed while the patient is still on-site could significantly improve the patient's quality of life.
aimWe develop and assess the feasibility of a one-hour-to-diagnosis (1Hr2Dx) method for processing and providing a preliminary diagnosis of a set of 12 prostate biopsies. APPROACH: We developed a fluorescence staining, optical clearing, and 3D open-top light-sheet microscopy workflow to enable 12 prostate needle core biopsies to be processed and diagnosed within an hour of receipt. We analyzed 44 biopsies by the 1Hr2Dx method, which does not consume tissue. The biopsies were then processed for routine, slide-based 2D histology. Three pathologists independently evaluated the 3D 1Hr2Dx and 2D slide-based datasets in a blinded, randomized fashion. Turnaround times were recorded, and the accuracy of our method was compared with gold-standard slide-based histology.
resultsThe average turnaround time for tissue processing, imaging, and diagnosis was 44.5 min. The sensitivity and specificity of 1Hr2Dx in diagnosing cancer were both >90 % .
conclusionsThe 1Hr2Dx method has the potential to improve patient care by providing an accurate preliminary diagnosis within an hour of biopsy.
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Registered trials
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