ArticleFrontiers in plant science2020
Turning Up the Temperature on CRISPR: Increased Temperature Can Improve the Editing Efficiency of Wheat Using CRISPR/Cas9.
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Improvement of simultaneous genome editing of homoeologous loci in polyploid wheat using CRISPR/Cas9 applying tRNA processing system.Plant biotechnology (Tokyo, Japan) · 2025Article
- How to use CRISPR/Cas9 in plants: from target site selection to DNA repair.Journal of experimental botany · 2024Review
- Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses.Frontiers in plant science · 2024Review
- Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery.Frontiers in plant science · 2024Article
- Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression.Plant biotechnology (Tokyo, Japan) · 2023Article
- Efficient gene activation in plants by the MoonTag programmable transcriptional activator.Nucleic acids research · 2023Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding.Plants (Basel, Switzerland) · 2023Review
- Efficient gene activation in plants by the MoonTag programmable transcriptional activator.bioRxiv : the preprint server for biology · 2023Article
- Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice.Frontiers in genome editing · 2023Article
- Temperature dependent in vitro binding and release of target DNA by Cas9 enzyme.Scientific reports · 2022Article
- Article
- Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects.Molecular biology reports · 2022Article
- The heat is on: a simple method to increase genome editing efficiency in plants.BMC plant biology · 2022Article
- Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Frontiers in plant science · 2022Article
- Wheat root systems as a breeding target for climate resilience.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Highly Efficient Generation of Canker-Resistant Sweet Orange Enabled by an Improved CRISPR/Cas9 System.Frontiers in plant science · 2021Article
- Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane.Frontiers in genome editing · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of CRISPR/Cas9 technologies has transformed our ability to target and edit designated regions of a genome. It's broad adaptability to any organism has led to countless advancements in our understanding of many biological processes. Many current tools are designed for simple plant systems such as diploid species, however, efficient deployment in crop species requires a greater efficiency of editing as these often contain polyploid genomes. Here, we examined the role of temperature to understand if CRISPR/Cas9 editing efficiency can be improved in wheat. The recent finding that plant growth under higher temperatures could increase mutation rates was tested with Cas9 expressed from two different promoters in wheat. Increasing the temperature of the tissue culture or of the seed germination and early growth phase increases the frequency of mutation in wheat when the Cas9 enzyme is driven by the
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