ArticleFrontiers in pharmacology2020
Species Quantification in Complex Herbal Formulas-Vector Control Quantitative Analysis as a New Method.
Article in Frontiers in pharmacology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Artificial intelligence and synthetic biology in traditional Chinese medicine: revolutionizing public health applications.Frontiers in plant science · 2026Review
- A novel algorithm- specific loci screening accelerates the establishment of molecular quantification of Glycyrrhiza glabra, G. uralensis, and G. inflata.Chinese medicine · 2025Article
- Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Heliyon · 2024Article
- Molecular quantification of fritillariae cirrhosae bulbus and its adulterants.Chinese medicine · 2024Article
- Phytochemistry and Biological Profile of the Chinese Endemic Herb GenusMolecules (Basel, Switzerland) · 2024Review
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Pharmacodynamic Mechanism of Kuanxiong Aerosol for Vasodilation and Improvement of Myocardial Ischemia.Chinese journal of integrative medicine · 2022Article
- Strategies for molecular authentication of herbal products: from experimental design to data analysis.Chinese medicine · 2022Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Product mislabeling and/or species fraud in Traditional Chinese Medicine (TCM) not only decrease TCM quality, but also pose a potential health issue to the end user. Up to now, methods to control TCM quality have been developed to detect specific metabolites or identify the original species. However, species quantification in complex herbal formulas is rarely concerned. Here, we reported a simple Vector Control Quantitative Analysis (VCQA) method for flexible and accurate multiplex species quantification in traditional Chinese herbal formulas. We developed PCR-based strategy to quickly generate the integrated DNA fragments from multiple targeted species, which can be assembled into the quantitative vector in one round of cloning by Golden Gate ligation and Gateway recombination technique. With this method, we recruited the nuclear ribosomal DNA Internal Transcribed Spacer (ITS) region for the quantification of
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