ArticleCommunications biology2020
Unequal contribution of two paralogous CENH3 variants in cowpea centromere function.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Retention of a single Cenp-C gene in different syntenic locations in the montium group of Drosophila species.Heredity · 2026Article
- Retention of a singlebioRxiv : the preprint server for biology · 2025Article
- Inter-subfamily cybrid plants between wheat and maize, Zeawheat: production via an in vitro fertilization system, genome composition, and photosynthetic type.Journal of experimental botany · 2025Article
- Chromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitor.Life science alliance · 2024Article
- Immunodetection of tubulin and centromeric histone H3 (CENH3) proteins in Glycine species.Molecular biology reports · 2024Article
- Centromere sequence-independent but biased loading of subgenome-specific CENH3 variants in allopolyploid Arabidopsis suecica.Plant molecular biology · 2024Article
- Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids.Frontiers in plant science · 2024Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Efficient multi-allelic genome editing via CRISPR-Cas9 ribonucleoprotein-based delivery toFrontiers in plant science · 2024Article
- A near-complete assembly of asparagus bean provides insights into anthocyanin accumulation in pods.Plant biotechnology journal · 2023Article
- Comparative analysis of repetitive DNA in dysploid and non-dysploid Phaseolus beans.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- Chemical emasculation in cowpea (Vigna unguiculata (L.) Walp.) and dicotyledonous model species using trifluoromethanesulfonamide (TFMSA).Plant reproduction · 2023Article
- Recurrent but Short-Lived Duplications of Centromeric Proteins in Holocentric Caenorhabditis Species.Molecular biology and evolution · 2022Article
- Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year.Plant methods · 2022Article
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- Epigenetically mismatched parental centromeres trigger genome elimination in hybrids.Science advances · 2021Article
- The origin and evolution of a two-component system of paralogous genes encoding the centromeric histone CENH3 in cereals.BMC plant biology · 2021Article
- Expression of Two Rye CENH3 Variants and Their Loading into Centromeres.Plants (Basel, Switzerland) · 2021Article
- BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris.Chromosoma · 2021Article
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Authors and funding
17 authors at 6 institutions in 7 countries.
Funding
Abstract
In most diploids the centromere-specific histone H3 (CENH3), the assembly site of active centromeres, is encoded by a single copy gene. Persistance of two CENH3 paralogs in diploids species raises the possibility of subfunctionalization. Here we analysed both CENH3 genes of the diploid dryland crop cowpea. Phylogenetic analysis suggests that gene duplication of CENH3 occurred independently during the speciation of Vigna unguiculata. Both functional CENH3 variants are transcribed, and the corresponding proteins are intermingled in subdomains of different types of centromere sequences in a tissue-specific manner together with the kinetochore protein CENPC. CENH3.2 is removed from the generative cell of mature pollen, while CENH3.1 persists. CRISPR/Cas9-based inactivation of CENH3.1 resulted in delayed vegetative growth and sterility, indicating that this variant is needed for plant development and reproduction. By contrast, CENH3.2 knockout individuals did not show obvious defects during vegetative and reproductive development. Hence, CENH3.2 of cowpea is likely at an early stage of pseudogenization and less likely undergoing subfunctionalization.
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