ArticleScientific reports2020
Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Validating the classics: Accurate reference gene panel for reliable RT-qPCR in Porifera.PloS one · 2026Article
- Article
- Reference Genes for Expression Analyses by qRT-PCR inBiology · 2025Article
- Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic AlkalithermophileMicroorganisms · 2025Article
- A-to-I RNA editing ofeLife · 2025Article
- Transposon accumulation at xenobiotic gene family loci in aphids.Genome research · 2023Article
- The Peculiarities ofInsects · 2023Article
- Reference Genes for Expression Analysis Using RT-qPCR inInsects · 2022Article
- Identification and Validation of Reference Genes for Expression Analysis Using qRT-PCR inInsects · 2022Article
- Evaluation of Reference Genes inGenes · 2021Article
- Selection and Validation of Reference Genes For qRT-PCR Analysis ofFrontiers in physiology · 2021Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metopolophium dirhodum (Walker) (Hemiptera: Aphididae) is one of the most common aphid pests of winter cereals. To facilitate accurate gene expression analyses with qRT-PCR assays, the expression stability of candidate reference genes under specific experimental conditions must be verified before they can be used to normalize target gene expression levels. In this study, 10 candidate reference genes in M. dirhodum were analyzed by qRT-PCR under various experimental conditions. Their expression stability was evaluated with delta Ct, BestKeeper, geNorm, and NormFinder methods, and the final stability ranking was determined with RefFinder. The results indicate that the most appropriate sets of internal controls were SDHB and RPL8 across geographic population; RPL8, Actin, and GAPDH across developmental stage; SDHB and NADH across body part; RPL8 and Actin across wing dimorphism and temperature; RPL4 and EF1A across starvation stress; AK and RPL4 across insecticide treatments; RPL8 and NADH across antibiotic treatments; RPL8, RPL4, Actin, and NADH across all samples. The results of this study provide useful insights for establishing a standardized qRT-PCR procedure for M. dirhodum and may be relevant for identifying appropriate reference genes for molecular analyses of related insects.
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