Evidence map›Paper›PMID 33319778›Full record

ArticleNature communications2020

A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity.

David Wylensek, Thomas C A Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R Lesker, Sara B Hernández and 36 more

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 146 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
146citing papers in PubMed, 2 pooled it
19.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

146 citing papers in PubMed, 2 syntheses or guidelines pooled it, 211 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
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  9. Effects ofAnimals : an open access journal from MDPI · 2026
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  10. Microorganisms · 2026
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  15. Article
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86 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

46 authors at 19 institutions in 8 countries.

David WylensekFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany.
Thomas C A HitchFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany.ORCID 0000-0003-2244-7412
Thomas RiedelLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Afrizal AfrizalFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany.
Neeraj KumarFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany.
Esther WortmannFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany.
Tianzhe LiuChair of Technical Biochemistry, Technical University of Dresden, Dresden, Germany.
Saravanan DevendranMicrobiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, IL, USA.
Till R LeskerDepartment of Microbial Immune Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Sara B HernándezLaboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, Umeå, Sweden.ORCID 0000-0002-8349-360X
Viktoria HeineLaboratory for Biomaterials, Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Eva M BuhlElectron Microscopy Facility, Institute of Pathology, RWTH University Hospital, Aachen, Germany.
Paul M D'AgostinoChair of Technical Biochemistry, Technical University of Dresden, Dresden, Germany.ORCID 0000-0002-8323-5416
Fabio CumboDepartment CIBIO, University of Trento, Trento, Italy.ORCID 0000-0003-2920-5838
Thomas FischöderLaboratory for Biomaterials, Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Marzena WyschkonLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Torey LooftNational Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.ORCID 0000-0001-8066-6715
Valeria R ParreiraDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Canada.
Birte AbtLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Heidi L DodenMicrobiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, IL, USA.
Lindsey LyMicrobiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, IL, USA.
João M P AlvesDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Markus ReichlinPharmaBiome AG, Zurich, Switzerland.
Krzysztof FlisikowskiChair of Livestock Biotechnology, Weihenstephan School of Life Science, Technical University of Munich, Freising, Germany.
Laura Navarro SuarezInstitute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Hamburg, Germany.
Anthony P NeumannDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.
Garret SuenDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0002-6170-711X
Tomas de WoutersPharmaBiome AG, Zurich, Switzerland.
Sascha RohnInstitute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Hamburg, Germany.
Ilias LagkouvardosZIEL - Institute for Food & Health, Technical University of Munich, Freising, Germany.
Emma Allen-VercoeDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Canada.ORCID 0000-0002-8716-327X
Cathrin SpröerLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Boyke BunkLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.ORCID 0000-0002-8420-8161
Anja J Taverne-ThieleHost-Microbe Interactomics Group, Department of Animal Science, Wageningen University, Wageningen, The Netherlands.
Marcel GiesbersElectron Microscopy Center, Wageningen University, Wageningen, The Netherlands.
Jerry M WellsHost-Microbe Interactomics Group, Department of Animal Science, Wageningen University, Wageningen, The Netherlands.
Klaus NeuhausZIEL - Institute for Food & Health, Technical University of Munich, Freising, Germany.ORCID 0000-0002-6020-2814
Angelika SchniekeZIEL - Institute for Food & Health, Technical University of Munich, Freising, Germany.
Felipe CavaLaboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, Umeå, Sweden.
Nicola SegataDepartment CIBIO, University of Trento, Trento, Italy.ORCID 0000-0002-1583-5794
Lothar EllingLaboratory for Biomaterials, Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-3654-0397
Till StrowigDepartment of Microbial Immune Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.ORCID 0000-0003-0185-1459
Jason M RidlonMicrobiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, IL, USA.
Tobias A M GulderChair of Technical Biochemistry, Technical University of Dresden, Dresden, Germany.
Jörg OvermannLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.ORCID 0000-0003-3909-7201
Thomas ClavelFunctional Microbiome Research Group, RWTH University Hospital, Aachen, Germany. tclavel@ukaachen.de.ORCID 0000-0002-7229-5595
Universitätsklinikum Aachen · DEGerman Center for Infection Research · DEUniversity of Illinois Urbana-Champaign · USRWTH Aachen University · DETechnische Universität Dresden · DEWageningen University & Research · NLLeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures · DELeibniz-Institute for Food Systems Biology at the Technical University of Munich · DEUmeå University · SEUniversität Hamburg · DEUniversity of Guelph · CAUniversity of Trento · ITUniversity of Wisconsin–Madison · USAgricultural Research Service · USHellenic Centre for Marine Research · GRHelmholtz Centre for Infection Research · DEMedizinische Hochschule Hannover · DETechnical University of Munich · DEUniversidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus, Fusobacterium, and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci, and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae. The strain collection, called 'Pig intestinal bacterial collection' (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota.

Indexed as

Gastrointestinal MicrobiomePhylogenyAged, 80 and overAnimalsBacteriaBile Acids and SaltsBiodiversityClostridiumFecesFemaleGenes, BacterialHost SpecificityHumansIntestinesMaleMetagenomeBile Acids and SaltsRNA, Ribosomal, 16S

Identifiers

PMID33319778
PMCPMC7738495
OpenAlexW3113375338

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.