ArticleeLife2020
Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons.Nucleic acids research · 2025Article
- Screening Key Sites of Class 2 Integron Integrase that Impact Recombination Efficiency.Current microbiology · 2024Article
- Karyotypic stasis and swarming influenced the evolution of viral tolerance in a species-rich bat radiation.Cell genomics · 2024Article
- Methods for the targeted sequencing and analysis of integrons and their gene cassettes from complex microbial communities.Microbial genomics · 2022Article
- The coordinated replication of Vibrio cholerae's two chromosomes required the acquisition of a unique domain by the RctB initiator.Nucleic acids research · 2021Article
- Joint effects of genes underlying a temperature specialization tradeoff in yeast.PLoS genetics · 2021Article
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Authors and funding
6 authors.
Funding
Abstract
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited. Integron integrases innovated from double-strand- toward single-strand-DNA recombination through the acquisition of the I2 α-helix. To investigate how this transition was possible, we have evolved integrase IntI1 to what should correspond to an early innovation state by selecting for its ancestral activity. Using synonymous alleles to enlarge sequence space exploration, we have retrieved 13 mutations affecting both I2 and the multimerization domains of IntI1. We circumvented epistasis constraints among them using a combinatorial library that revealed their individual and collective fitness effects. We obtained up to 10
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