ArticleMaterials today. Bio2020
Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step.
Article in Materials today. Bio, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.Frontiers in bioengineering and biotechnology · 2023Review
- Supported Ionic Liquids Used as Chromatographic Matrices in Bioseparation-An Overview.Molecules (Basel, Switzerland) · 2022Review
- Efficient Isolation of Bacterial RNAs Using Silica-Based Materials Modified with Ionic Liquids.Life (Basel, Switzerland) · 2021Article
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8 authors.
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Abstract
Nucleic acids are relevant biopolymers in therapy and diagnosis, for which their purity and biological activity are of crucial relevance. However, these features are difficult to achieve by cost-effective methods. Herein, we report the functionalization of a macroporous chromatographic support functionalized with an ionic liquid (IL) with remarkable performance to purify nucleic acids. An initial screening with distinct IL chemical structures supported in silica was carried out, allowing to identify the IL 1-methyl-3-propylimidazolium chloride as the most promising ligand. A chromatographic macroporous matrix able to be used in preparative liquid chromatography was then functionalized and binding/elution studies were performed. The IL 1-methyl-3-propylimidazolium chloride acts as a multimodal ligand with a remarkable dynamic binding capacity. This macroporous support allows the (one-step) purification of nucleic acids, namely small RNAs, ribosomal RNA, and genomic DNA, from a bacterial lysate, and can be regenerated and reused without compromising its separation performance.
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