ArticleScientific reports2020
Characterization of Enterococcus faecalis in different culture conditions.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
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- Widespread dissemination of the RepA_N-type megaplasmid conferring a carbohydrate utilization advantage in clonal complex 17Microbiology spectrum · 2026Article
- Temporal variability is an inherent feature of the healthy canine microbiome assessed by full-length 16S rRNA gene sequencing.Animal microbiome · 2026Article
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- A New Disinfection Approach Using a Chitosan-Based Endodontic Irrigant.Materials (Basel, Switzerland) · 2025Article
- Hidden diversity inMicrobiology spectrum · 2025Article
- Label-free rapid antimicrobial susceptibility testing with machine-learning based dynamic holographic laser speckle imaging.Biosensors & bioelectronics · 2025Article
- Bioactive Compound Characterization and Phytopharmacological Potentials of Tulbaghia violacea Fruits and Seeds.Chemistry & biodiversity · 2025Article
- Sucrose reduces biofilm formation byBiofilm · 2025Article
- Green synthesis of silver NPs using aqueous extract ofFrontiers in microbiology · 2025Article
- A Comparative In-Vitro Study of the Effectiveness of Several Methods of Sterilizing Endodontic Files.Cureus · 2024Article
- Identification of Enterococcus spp. by MALDI-TOF mass spectrometry isolated from clinical mastitis and bulk tank milk samples.BMC veterinary research · 2024Article
- CRISPR-Cas System, Antimicrobial Resistance, andBiomedicines · 2024Article
- Medical Device-Associated Biofilm Infections and Multidrug-Resistant Pathogens.Pathogens (Basel, Switzerland) · 2024Review
- The Influence of L-Lysine-Alpha-Oxidase on the Biofilm Formation of Opportunistic Microorganisms Associated with Inflammatory Diseases of the Urinary Tract.Pathogens (Basel, Switzerland) · 2024Article
- The role of metagenomic next-generation sequencing in diagnosing and managing post-kidney transplantation infections.Frontiers in cellular and infection microbiology · 2024Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate how carbohydrates (glucose or sucrose) affect the characteristics of Enterococcus faecalis (E. faecalis) planktonic and biofilm in vitro. For this study, E. faecalis was cultured in tryptone-yeast extract broth with 0% glucose + 0% sucrose, 0.5% glucose, 1% glucose, 0.5% sucrose, or 1% sucrose. Viability of E. faecalis was examined by colony forming unit counting assays. Biofilm formation was assessed by measuring extracellular DNA (eDNA), a component of the biofilm matrix. Quantitative real-time PCR (qRT-PCR) was performed to investigate the expression of virulence-associated genes. Field emission scanning electron microscopy analysis, confocal laser scanning microscopy analysis, and crystal violet colorimetric assay were conducted to study E. faecalis biofilms. E. faecalis showed the highest viability and eDNA levels in 1% sucrose medium in biofilms. The result of qRT-PCR showed that the virulence-associated genes expressed highest in 1% sucrose-grown biofilms and in 1% glucose-grown planktonic cultures. E. faecalis showed highly aggregated biofilms and higher bacteria and exopolysaccharide (EPS) bio-volume in sucrose than in 0% glucose + 0% sucrose or glucose. The results indicate that the production of eDNA and EPS and expression of virulence-associated genes in E. faecalis are affected by the concentration of carbohydrates in biofilm or planktonic culture.
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