ArticleProceedings of the National Academy of Sciences of the United States of America2020
Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- Engineering a photoactivatable A-to-I RNA base editor for gene therapy in vivo.Nature biotechnology · 2026Article
- Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli.Journal of biological engineering · 2025Article
- A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing inJournal of microbiology and biotechnology · 2025Article
- A noninvasive photoactivatable split-Cre recombinase system for genome engineering in zebrafish.iScience · 2024Article
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.Cell structure and function · 2023Article
- Synthetic peptides for the precise transportation of proteins of interests to selectable subcellular areas.Frontiers in bioengineering and biotechnology · 2023Article
- Stable Transgenic Mouse Strain with Enhanced Photoactivatable Cre Recombinase for Spatiotemporal Genome Manipulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Optogenetics for transcriptional programming and genetic engineering.Trends in genetics : TIG · 2022Review
- Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles.Biotech (Basel (Switzerland)) · 2022Article
- The expanding role of split protein complementation in opsin-free optogenetics.Current opinion in pharmacology · 2022Review
- Optogenetic Application to Investigating Cell Behavior and Neurological Disease.Frontiers in cellular neuroscience · 2022Review
Corrections and comments
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise genetic engineering in specific cell types within an intact organism is intriguing yet challenging, especially in a spatiotemporal manner without the interference caused by chemical inducers. Here we engineered a photoactivatable Dre recombinase based on the identification of an optimal split site and demonstrated that it efficiently regulated transgene expression in mouse tissues spatiotemporally upon blue light illumination. Moreover, through a double-floxed inverted open reading frame strategy, we developed a Cre-activated light-inducible Dre (CALID) system. Taking advantage of well-defined cell-type-specific promoters or a well-established Cre transgenic mouse strain, we demonstrated that the CALID system was able to activate endogenous reporter expression for either bulk or sparse labeling of CaMKIIα-positive excitatory neurons and parvalbumin interneurons in the brain. This flexible and tunable system could be a powerful tool for the dissection and modulation of developmental and genetic complexity in a wide range of biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.