ArticleBiotechnology for biofuels2020
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins.
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor-Like Performance.Biotechnology and bioengineering · 2026Article
- Engineering ofMicroorganisms · 2026Article
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- Efficient Expression and Characterization of an Endo-Type Lyase HCLase_M28 and Its Gradual Scale-Up Fermentation for the Preparation of Chondroitin Sulfate Oligosaccharides.Applied biochemistry and biotechnology · 2024Article
- CAT Bridge: an efficient toolkit for gene-metabolite association mining from multiomics data.GigaScience · 2024Article
- Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.World journal of microbiology & biotechnology · 2023Review
- Evaluation of VariousJournal of microbiology and biotechnology · 2023Article
- Current Trends in Acetins Production: Green versus Non-Green Synthesis.Molecules (Basel, Switzerland) · 2022Review
- Glutamate as a non-conventional substrate for high production of the recombinant protein inFrontiers in microbiology · 2022Article
- Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic Engineering of Central Carbon Metabolism of Bacillus licheniformis for Enhanced Production of Poly-γ-glutamic Acid.Applied biochemistry and biotechnology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundThe economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli.
resultsThis issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively.
conclusionsThe result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged.
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Registered trials
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