Evidence map›Paper›PMID 33315501›Full record

ArticleEpigenetics2021

Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation.

Issam M Mayyas, Robert J Weeks, Robert C Day, Helena E Magrath, Karina M O'Connor, Olga Kardailsky, Timothy A Hore, Mark B Hampton, Ian M Morison

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. The Dual Role ofBiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Issam M MayyasDepartment of Pathology, University of Otago, Dunedin, New Zealand.
Robert J WeeksDepartment of Pathology, University of Otago, Dunedin, New Zealand.ORCID 0000-0003-0474-9089
Robert C DayDepartment of Biochemistry, University of Otago, Dunedin, New Zealand.ORCID 0000-0002-0043-5149
Helena E MagrathDepartment of Pathology, University of Otago, Dunedin, New Zealand.
Karina M O'ConnorDepartment of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Olga KardailskyDepartment of Anatomy, University of Otago, Dunedin, New Zealand.
Timothy A HoreDepartment of Anatomy, University of Otago, Dunedin, New Zealand.ORCID 0000-0002-6735-225X
Mark B HamptonDepartment of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Ian M MorisonDepartment of Pathology, University of Otago, Dunedin, New Zealand.
University of Otago · NZUniversity of Pecs · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylation following treatment of cells by the DNA methyltransferase 1 (DNMT1) inhibitor, decitabine. As expected, we demonstrate complete loss of methylation on the daughter strand following S-phase in selected densely methylated genes in synchronized Jurkat cells. Thereafter, cells showed a heterogeneous pattern of methylation reflecting replication of the unmethylated strand and restoration of methylation.

Indexed as

DNA DemethylationDNA MethylationAzacitidineDecitabineDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesHumansSulfitesAzacitidineDecitabineDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-Methyltransferaseshydrogen sulfiteSulfitesdecitabineDemethylationdnmt1 inhibition

Identifiers

PMID33315501
PMCPMC8813075
OpenAlexW3111129374

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.