ArticleeLife2020
Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 35 citations in OpenAlex.
- Spatially guided in vivo single-cell functional genomics of postnatal heart.Nature cardiovascular research · 2026Article
- Rhythms of growth: unveiling the mechanobiology behind heart maturation.The Journal of physiology · 2026Review
- Cardiac epigenome in heart development and disease.Nature reviews. Cardiology · 2026Review
- G1/S arrest: a key mechanism of cellular aging and replicative senescence.Biogerontology · 2026Review
- Integrated expression quantitative trait loci and Mendelian randomization analyses of the candidate genes and pathways identified for myocardial infarction.Frontiers in molecular biosciences · 2026Article
- Distinct endothelial gene responses to acute exercise in skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2025Article
- Article
- The molecular landscape of vascular cells in the human brain.Nature reviews. Cardiology · 2024Article
- Brain resident microglia in Alzheimer's disease: foe or friends.Inflammopharmacology · 2024Review
- Generation and characterisation of scalable and stable human pluripotent stem cell-derived microvascular-like endothelial cells for cardiac applications.Angiogenesis · 2024Article
- Transcriptome analysis of cardiac endothelial cells after myocardial infarction reveals temporal changes and long-term deficits.Scientific reports · 2024Article
- Control of cardiac contractions using Cre-lox and degron strategies in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Vascular endothelial cell development and diversity.Nature reviews. Cardiology · 2023Review
- Increased Type I interferon signaling and brain endothelial barrier dysfunction in an experimental model of Alzheimer's disease.Scientific reports · 2022Article
- Translational potential of hiPSCs in predictive modeling of heart development and disease.Birth defects research · 2022Review
- Endothelial Lipid Metabolism.Cold Spring Harbor perspectives in medicine · 2022Review
- Beyond Static Pipes: Mechanisms and In Vitro Models of Vascular Aging.Cold Spring Harbor perspectives in medicine · 2022Review
- Why is endothelial resilience key to maintain cardiac health?Basic research in cardiology · 2022Review
- Assessment of systemic and ocular inflammation in juvenile idiopathic arthritis via choroidal vascularity index.Rheumatology international · 2022Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Endothelial cells (ECs) are widely heterogenous depending on tissue and vascular localization. Jambusaria et al. recently demonstrated that ECs in various tissues surprisingly possess mRNA signatures of their underlying parenchyma. The mechanism underlying this observation remains unexplained, and could include mRNA contamination during cell isolation, in vivo mRNA paracrine transfer from parenchymal cells to ECs, or cell-autonomous expression of these mRNAs in ECs. Here, we use a combination of bulk RNASeq, single-cell RNASeq datasets, in situ mRNA hybridization, and most importantly ATAC-Seq of FACS-isolated nuclei, to show that cardiac ECs actively express cardiomyocyte myofibril (CMF) genes and have open chromatin at CMF gene promoters. These open chromatin sites are enriched for sites targeted by cardiac transcription factors, and closed upon expansion of ECs in culture. Together, these data demonstrate unambiguously that the expression of CMF genes in ECs is cell-autonomous, and not simply a result of technical contamination or paracrine transfers of mRNAs, and indicate that local cues in the heart in vivo unexpectedly maintain fully open chromatin in ECs at genes previously thought limited to cardiomyocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.