ArticleMolecular plant pathology2021
Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.
Article in Molecular plant pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
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- Virulence Regulation and Lifestyle Transitions: The Role of c-di-GMP and Two-Component Systems in Erwinia amylovora and Their Evolutionary Context Within Enterobacterales.Molecular plant pathology · 2026Review
- From environmental signals to adaptive phenotypes: signal-responsive regulation and network logic of bacterial small RNAs.FEMS microbiology reviews · 2026Review
- Antagonistic Activity and Genomic Insights of Bacillus velezensis JE250 against Erwinia amylovora.The plant pathology journal · 2025Article
- Article
- Copper-induced stress mechanisms inApplied and environmental microbiology · 2025Article
- The RNA Chaperone Hfq and Small Non-Coding RNAs Modulate the Biofilm Formation of the Fish PathogenInternational journal of molecular sciences · 2025Article
- Evaluation of pathogenicity variation between twoFrontiers in microbiology · 2025Article
- Biofilm-Forming Ability of Phytopathogenic Bacteria: A Review of its Involvement in Plant Stress.Plants (Basel, Switzerland) · 2023Review
- Regulation of biofilm formation by non-coding RNA in prokaryotes.Current research in pharmacology and drug discovery · 2023Review
- A versatile TnPlant communications · 2022Article
- Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.Molecular plant pathology · 2021Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erwinia amylovora is the causative agent of the devastating disease fire blight of pome fruit trees. After infection of host plant leaves at apple shoot tips, E. amylovora cells form biofilms in xylem vessels, restrict water flow, and cause wilting symptoms. Although E. amylovora is well known to be able to cause systemic infection, how biofilm cells of E. amylovora transit from the sessile mode of growth in xylem to the planktonic mode of growth in cortical parenchyma remains unknown. Increasing evidence has suggested the important modulatory roles of Hfq-dependent small RNAs (sRNAs) in the pathogenesis of E. amylovora. Here, we demonstrate that the sRNA RprA acts as a positive regulator of amylovoran exopolysaccharide production, the type III secretion system (T3SS), and flagellar-dependent motility, and as a negative regulator of levansucrase activity and cellulose production. We also show that RprA affects the promoter activity of multiple virulence factor genes and regulates hrpS, a critical T3SS regulator, at the posttranscriptional level. We determined that rprA expression can be activated by the Rcs phosphorelay, and that expression is active during T3SS-mediated host infection in an immature pear fruit infection model. We further showed that overexpression of rprA activated the in vitro dispersal of E. amylovora cells from biofilms. Thus, our investigation of the varied role of RprA in affecting E. amylovora virulence provides important insights into the functions of this sRNA in biofilm control and systemic infection.
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