ArticlePest management science2021
An evaluation of Bacillus thuringiensis israelensis (AM65-52) treatment for the control of Aedes aegypti using vehicle-mounted WALS® application in a densely populated urban area of Puerto Rico.
Article in Pest management science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review of Aedes aegypti control trials suggests publication bias related to author disclosure of conflicts of interest.PLoS neglected tropical diseases · 2026Pooled it
- Strategies for controllingCaribbean journal of science · 2026Article
- Larviciding for malaria control and elimination in Africa.Malaria journal · 2025Review
- Dengue vector control in high-income, city settings: A scoping review of approaches and methods.PLoS neglected tropical diseases · 2024Article
- Acceptability of emergent Aedes aegypti vector control methods in Ponce, Puerto Rico: A qualitative assessment.PLOS global public health · 2024Article
- Community perceptions on challenges and solutions to implement an Aedes aegypti control project in Ponce, Puerto Rico (USA).PloS one · 2023Article
- Effectiveness of Aedes-borne infectious disease control in Latin America and the Caribbean region: A scoping review.PloS one · 2022Article
- Field evaluation of WALS truck-mounted A1 super duty mist sprayer® with VectoBac® WDG againstSN applied sciences · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWith a shortage of effective options for control of Aedes aegypti in Puerto Rico due to widespread resistance to conventional mosquito adulticides, an alternative approach was investigated to reduce vector populations. In two areas (totaling 144 ha) of the municipality of Bayamón, Puerto Rico, Bacillus thuringiensis israelensis (Bti) AM65-52 WDG was applied at a rate of 500 g/ha using vehicle-mounted aqueous wide-area larvicide spray applications weekly for 4 weeks and then every other week for a further 16 weeks. Bioassay jars were placed in the field to monitor for deposition of Bti droplets in open spaces, and under vegetation and building coverage. Autocidal gravid ovitraps were placed throughout the field site to monitor the population of adult female Ae. aegypti in both treatment and control sites.
resultsLarvicide spray was successfully deposited into jars in an array of open and covered locations, as confirmed by larval bioassays. After the fourth weekly spraying, differences in autocidal gravid ovitrap densities were observed between treatment and control sites resulting in 62% (P = 0.0001) and 28% (P < 0.0001) reductions in adult female Ae. aegypti numbers.
conclusionRepeated wide-area larvicide spray application of Bti AM65-52 WDG to residential areas in Puerto Rico effectively suppressed dengue vector populations. The success of this trial has led to expansion of the WALS® program to a larger area of Bayamón and other municipalities in Puerto Rico. © 2020 Society of Chemical Industry.
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