ArticleCurrent research in microbial sciences2020
Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins.
Article in Current research in microbial sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- Mechanisms of microbial co-aggregation in mixed anaerobic cultures.Applied microbiology and biotechnology · 2024Review
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Development and utilization of new OMicrobiology spectrum · 2024Article
- Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules.Nature protocols · 2023Review
- A Single-Site Mutation Tunes Fluorescence and Chromophorylation of an Orange Fluorescent Cyanobacteriochrome.Chembiochem : a European journal of chemical biology · 2023Article
- Zinc-Induced Fluorescence Turn-On in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins.Biochemistry · 2023Article
- Zinc-Induced Fluorescence Turn-on in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins.bioRxiv : the preprint server for biology · 2023Article
- Outer Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in LiveBiosensors · 2023Article
- Anaerobic fluorescent reporters for live imaging ofFrontiers in microbiology · 2023Article
- Using fluorescent promoter-reporters to study sugar utilization control in Bifidobacterium longum NCC 2705.Scientific reports · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Enhanced small green fluorescent proteins as a multisensing platform for biosensor development.Frontiers in bioengineering and biotechnology · 2022Article
- New Orange Ligand-Dependent Fluorescent Reporter for Anaerobic Imaging.ACS chemical biology · 2021Article
- Characterization of flavin binding in oxygen-independent fluorescent reporters.AIChE journal. American Institute of Chemical Engineers · 2020Article
- Nanaerobic growth enables direct visualization of dynamic cellular processes in human gut symbionts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent tools such as green fluorescent protein (GFP) have been used extensively as reporters in biochemistry and microbiology, but GFP and other conventional fluorescent proteins are restricted to aerobic environments. This limitation precludes fluorescence studies of anaerobic ecologies including polymicrobial communities in the human gut microbiome and in soil microbiomes, which profoundly affect health, disease, and the environment. To address this limitation, we describe the first implementation of two bilin-binding fluorescent proteins (BBFPs), UnaG and IFP2.0, as oxygen-independent fluorescent labels for live-cell imaging in anaerobic bacteria. Expression of UnaG or IFP2.0 in the prevalent gut bacterium
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.