ArticleAmerican journal of translational research2020
β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway.
Article in American journal of translational research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Pyroptosis was suppressed by 20-hydroxyecdysone through Lin28b-mediated let-7d in vascular endothelial cells.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Ecdysterone Alleviates Atherosclerosis by Inhibiting NCF2 and Inhibiting Ferroptosis Mediated by the PI3K/Akt/Nrf2 Pathway.Journal of cellular and molecular medicine · 2025Article
- Modulating Autophagy in Osteoarthritis: Exploring Emerging Therapeutic Drug Targets.International journal of molecular sciences · 2024Review
- Potential Effects of Indomethacin on Alleviating Osteoarthritis ProgressionJournal of musculoskeletal & neuronal interactions · 2024Article
- Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition.Journal of orthopaedic surgery and research · 2024Article
- Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis.Genes & diseases · 2023Article
- Mulberroside A alleviates osteoarthritis via restoring impaired autophagy and suppressing MAPK/NF-κB/PI3K-AKT-mTOR signaling pathways.iScience · 2023Article
- Identification of quality markers forPeerJ · 2023Article
- Autophagy in the pathogenesis and therapeutic potential of post-traumatic osteoarthritis.Burns & trauma · 2023Review
- LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral.Oncology research · 2023Article
- Rongjin Niantong Fang ameliorates cartilage degeneration by regulating the SDF-1/CXCR4-p38MAPK signalling pathway.Pharmaceutical biology · 2022Article
- Ozone induces autophagy by activating PPARγ/mTOR in rat chondrocytes treated with IL-1β.Journal of orthopaedic surgery and research · 2022Article
- Small molecules of herbal origin for osteoarthritis treatment: in vitro and in vivo evidence.Arthritis research & therapy · 2022Review
- Curcumin ameliorates HExperimental and therapeutic medicine · 2022Article
- The genusFrontiers in pharmacology · 2022Review
- Madecassic Acid Ameliorates the Progression of Osteoarthritis: An in vitro and in vivo Study.Drug design, development and therapy · 2022Article
- A bibliometrics analysis and visualization of osteoimmunology on osteoarthritis studies.American journal of translational research · 2022Article
- Regulation of osteoblast autophagy based on PI3K/AKT/mTOR signaling pathway study on the effect of β-ecdysterone on fracture healing.Journal of orthopaedic surgery and research · 2021Article
- Identification of Key Genes and Pathways in Osteoarthritis via Bioinformatic Tools: An Updated Analysis.Cartilage · 2021Article
- Loss of Autophagy Causes Increased Apoptosis of Tibial Plateau Chondrocytes in Guinea Pigs with Spontaneous Osteoarthritis.Cartilage · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the therapeutic effects of β-ecdysterone on osteoarthritis (OA) and the underlying mechanism.
methodsOA model was established on rats by injecting MIA. ELSA was used to determine the concentration of IL-1β, IL-6, NO and TNF-α in the chondrocytes and cartilage tissues. Immunofluorescence assay was used to determine the expression of collagen II in the chondrocytes. The survival rate of chondrocytes was evaluated by MTT assay. The apoptosis of chondrocytes was checked by AO/PI staining and flow cytometry assay. The expression level of Atg7, PI3K and caspase-3 was evaluated by qRT-PCR. Western Blot was used determine the expression of PI3K, p-AKT1, AKT1, p-mTOR, mTOR, p70S6K, p-p70S6K, LC3I, LC3II and caspase-3. HE staining was used to check the pathological state of cartilage tissues.
resultsChondrocytes were tolerable to rapamycin, 3-methyladenine and β-ecdysterone at the concentration of 10 mM, 100 nM and 40 μM, respectively. The apoptosis of chondrocytes was inhibited by rapamycin and β-ecdysterone, and induced by 3-methyladenine. PI3K, p-AKT1, p-mTOR, p-p70S6K and caspase-3 were down-regulated by rapamycin and β-ecdysterone, and up-regulated by 3-methyladenine in both the chondrocytes and the cartilage tissues. The expression of Atg7 and LC3II/LC3I were regulated in a opposite way. The inflammation state was improved by rapamycin and β-ecdysterone both the chondrocytes and the cartilage tissues. HE staining results showed that the pathological state of cartilage tissues was alleviated by β-ecdysterone.
conclusionβ-ecdysterone might alleviate osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway.
Indexed as
Identifiers
33312358PMC7724317What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.