Evidence map›Paper›PMID 33311493›Full record

ArticleNature communications2020

Receptor-targeted engineered probiotics mitigate lethal Listeria infection.

Rishi Drolia, Mary Anne Roshni Amalaradjou, Valerie Ryan, Shivendra Tenguria, Dongqi Liu, Xingjian Bai, Luping Xu, Atul K Singh, Abigail D Cox, Victor Bernal-Crespo and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed, 1 pooled it
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Review
  10. Foods (Basel, Switzerland) · 2025
    Article
  11. Article
  12. Review
  13. Review
  14. StreamliningAntibiotics (Basel, Switzerland) · 2025
    Article
  15. Article
  16. Article
  17. Review
  18. Whole Genome Analysis ofAntibiotics (Basel, Switzerland) · 2025
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Rishi DroliaMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-8405-2961
Mary Anne Roshni AmalaradjouMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Valerie RyanMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Shivendra TenguriaMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Dongqi LiuMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Xingjian BaiMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Luping XuMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Atul K SinghMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Abigail D CoxDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.ORCID 0000-0003-2262-8168
Victor Bernal-CrespoDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-7161-3600
James A SchaberBindley Bioscience Research Center, Purdue University, West Lafayette, IN, USA.
Bruce M ApplegateMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.
Ramesh VemulapalliDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-2473-2906
Arun K BhuniaMolecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA. bhunia@purdue.edu.ORCID 0000-0003-3640-1554
Purdue University West Lafayette · USUniversity of Connecticut · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Probiotic bacteria reduce the intestinal colonization of pathogens. Yet, their use in preventing fatal infection caused by foodborne Listeria monocytogenes (Lm), is inconsistent. Here, we bioengineered Lactobacillus probiotics (BLP) to express the Listeria adhesion protein (LAP) from a non-pathogenic Listeria (L. innocua) and a pathogenic Listeria (Lm) on the surface of Lactobacillus casei. The BLP strains colonize the intestine, reduce Lm mucosal colonization and systemic dissemination, and protect mice from lethal infection. The BLP competitively excludes Lm by occupying the surface presented LAP receptor, heat shock protein 60 and ameliorates the Lm-induced intestinal barrier dysfunction by blocking the nuclear factor-κB and myosin light chain kinase-mediated redistribution of the major epithelial junctional proteins. Additionally, the BLP increases intestinal immunomodulatory functions by recruiting FOXP3

Indexed as

Administration, OralAnimalsBacterial AdhesionBacterial ProteinsCD11c AntigenCell LineChaperonin 60Dendritic CellsDisease Models, AnimalFemaleForkhead Transcription FactorsHumansIntestinesKiller Cells, NaturalLacticaseibacillus caseiListeriaBacterial ProteinsCD11c AntigenChaperonin 60Forkhead Transcription FactorsFoxp3 protein, mouseMyosin-Light-Chain KinaseNF-kappa B

Identifiers

PMID33311493
PMCPMC7732855
OpenAlexW3110879748

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.