ArticleMicrobial cell factories2020
Engineering the Calvin-Benson-Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 80 citations in OpenAlex.
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- Diversity and Potential Metabolic Characteristics of Culturable Copiotrophic Bacteria That Can Grow on Low-Nutrient Medium in Zhenbei Seamount in the South China Sea.Microbial ecology · 2024Article
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
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- Overexpression of RuBisCO form I and II genes inApplied and environmental microbiology · 2024Article
- Role of the cathode chamber in microbial electrosynthesis: A comprehensive review of key factors.Engineering microbiology · 2024Review
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Polyhydroxyalkanoate Copolymer Production by RecombinantBioengineering (Basel, Switzerland) · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- COMicrobial cell factories · 2024Article
- Soil microbial COFrontiers in microbiology · 2024Article
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.Bioresources and bioprocessing · 2023Article
- Prospects for engineeringEngineering microbiology · 2023Review
- Trends in Research and Development for COACS omega · 2023Review
- Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from COMicrobial cell factories · 2022Article
- Production of biopolymer precursors beta-alanine and L-lactic acid from COFrontiers in bioengineering and biotechnology · 2022Article
- A Review on EnhancingFrontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCO
resultsIn this work, the autotrophic microbe Ralstonia eutropha (Cupriavidus necator) was selected as a research platform for CBB cycle optimization engineering. By knocking out either CBB operon genes on the operon or mega-plasmid of R. eutropha, we found that both CBB operons were active and contributed almost equally to the carbon fixation process. With similar knock-out experiments, we found both soluble and membrane-bound hydrogenases (SH and MBH), belonging to the energy providing hydrogenase module, were functional during autotrophic growth of R. eutropha. SH played a more significant role. By introducing a heterologous cyanobacterial RuBisCO with the endogenous GroES/EL chaperone system(A quality control systems for proteins consisting of molecular chaperones and proteases, which prevent protein aggregation by either refolding or degrading misfolded proteins) and RbcX(A chaperone in the folding of Rubisco), the culture OD
conclusionsTo our best knowledge, this is the first report of successfully engineering the CBB pathway and hydrogenases of R. eutropha for improved activity, and is one of only a few cases where the efficiency of CO
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