ArticleJournal of structural biology2021
Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block.
Article in Journal of structural biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Stoichiometry and architecture of the human pyruvate dehydrogenase complex.Science advances · 2024Article
- Dimerization of a 5-kDa domain defines the architecture of the 5-MDa gammaproteobacterial pyruvate dehydrogenase complex.Science advances · 2024Article
- A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.Journal of structural biology · 2023Article
- Developing Graphene Grids for Cryoelectron Microscopy.Frontiers in molecular biosciences · 2022Review
- Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation.International journal of molecular sciences · 2021Review
- Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block.Journal of visualized experiments : JoVE · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Support films are commonly used during cryo-EM specimen preparation to both immobilise the sample and minimise the exposure of particles at the air-water interface. Here we report preparation protocols for carbon and graphene supported single particle electron microscopy samples using a novel 3D-printed sample transfer block to facilitate the direct, wetted, movement of both carbon and graphene supports from the substrate on which they were generated to small volumes (10 μL) of sample. These approaches are simple and inexpensive to implement, minimise hydrophobic contamination of the support films, and are widely applicable to single particle studies. Our approach also allows the direct exchange of the sample buffer on the support film in cases in which it is unsuitable for vitrification, e.g. for samples from centrifugal density gradients that help to preserve sample integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.