Evidence map›Paper›PMID 33306280›Full record

ArticleMicrobiologyOpen2020

Bacterial-like nitric oxide synthase in the haloalkaliphilic archaeon Natronomonas pharaonis.

Silvia S Orsini, Kimberly L James, Destiny J Reyes, Ricardo L Couto-Rodriguez, Miriam K Gulko, Angela Witte, Ronan K Carroll, Kelly C Rice

Open access · goldAbstract read
In one paragraph

Article in MicrobiologyOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Hydrostatic pressure-enabled transformation inFrontiers in microbiology · 2026
    Article
  2. Review
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  4. Review
  5. Review
  6. Virulence ofFrontiers in immunology · 2021
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Silvia S OrsiniDepartment of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
Kimberly L JamesDepartment of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
Destiny J ReyesDepartment of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
Ricardo L Couto-RodriguezDepartment of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
Miriam K GulkoDepartment Oesterhelt, Max Planck Institut für Biochemie, Martinsried, Germany.
Angela WitteDepartment of Microbiology, Immunobiology and Genetics, MPL Laboratories, University of Vienna, Vienna, Austria.
Ronan K CarrollDepartment of Biological Sciences, Ohio University, Athens, OH, USA.
Kelly C RiceDepartment of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.ORCID 0000-0003-1335-4409
University of Florida · USMax Planck Institute of Biochemistry · DEOhio University · USUniversity of Vienna · AT

Funding

Regulation of Staphylococcus aureus physiology by bacterial nitric oxideR01AI118999 · NIAID · UNIVERSITY OF FLORIDA · PI RICE, KELLY CHRISTINE · 2016 to 2020
$1.7M
NIAID NIH HHS R01 AI118999
6 · The paper itself

Abstract

Bacterial nitric oxide (NO) synthases (bNOS) play diverse and important roles in microbial physiology, stress resistance, and virulence. Although bacterial and mammalian NOS enzymes have been well-characterized, comparatively little is known about the prevalence and function of NOS enzymes in Archaea. Analysis of archaeal genomes revealed that highly conserved bNOS homologs were restricted to members of the Halobacteria. Of these, Natronomonas pharaonis NOS (npNOS) was chosen for further characterization. NO production was confirmed in heterologously expressed His-tagged npNOS by coupling nitrite production from N-hydroxy-L-arginine in an H

Indexed as

AcetatesEscherichia coliGenome, ArchaealHalobacteriaceaeHydrogen PeroxideNitric OxideNitric Oxide SynthaseOxidation-ReductionOxygen ConsumptionAcetatesHydrogen PeroxideNitric OxideNitric Oxide Synthase

Identifiers

PMID33306280
PMCPMC7658456
OpenAlexW3093294645

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.