ArticleAMB Express2020
High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Establishment of an Indirect ELISA Detection Method for Porcine Circovirus 3 Based on Soluble Cap Protein.Veterinary sciences · 2026Article
- Immunogenicity Analysis of PCV3 Capsid Highly Expressed Using Baculovirus.International journal of molecular sciences · 2026Article
- Epidemiological Survey of Porcine Circovirus Types 2 and 3 in Liaoning Region of China and Preparation of Monoclonal Antibodies Against PCV3 Cap Protein.Veterinary sciences · 2026Article
- The porcine circovirus 3 humoral response: characterization of maternally derived antibodies and dynamic following experimental infection.Microbiology spectrum · 2024Article
- Molecular characterization of canine circovirus based on the Capsid gene in Thailand.BMC veterinary research · 2024Article
- The PCV3 Cap Virus-like Particle Vaccine with the Chimeric PCV2-Neutralizing Epitope Gene Is Effective in Mice.Veterinary sciences · 2024Article
- Recombinant expression and characterization of Canine circovirus capsid protein for diagnosis.Frontiers in veterinary science · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
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Registered trials
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