ArticleThe Journal of cell biology2021
An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.
Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Emerin loss of function inhibits MyoD-driven differentiation of human iPSCs into skeletal myotubes.bioRxiv : the preprint server for biology · 2026Article
- Distinct classes of lamina-associated domains are defined by differential patterns of repressive histone methylation.Genome research · 2025Article
- Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen.bioRxiv : the preprint server for biology · 2025Article
- Techniques to detect physical interactions between genomic regions.Epigenomics · 2025Review
- APEX2 RNA Proximity Labeling in Mammalian Cell Lines With Low Biotin Permeability.Bio-protocol · 2025Article
- Review
- SMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins.JACS Au · 2025Article
- Laboratory Evolution of Metalloid Reductase Substrate Recognition and Nanoparticle Product Size.ACS chemical biology · 2024Article
- The catalytic subunit of DNA-PK regulates transcription and splicing of AR in advanced prostate cancer.The Journal of clinical investigation · 2023Article
- Chemical and Biological Strategies for Profiling Protein-Protein Interactions in Living Cells.Chemistry, an Asian journal · 2023Review
- GATS tag system is compatible with biotin labelling methods for protein analysis.Scientific reports · 2023Article
- PP2A methylesterase PME-1 suppresses anoikis and is associated with therapy relapse of PTEN-deficient prostate cancers.Molecular oncology · 2023Article
- High quality mapping of chromatin at or near the nuclear lamina from small numbers of cells reveals cell cycle and developmental changes of chromatin at the nuclear periphery.Nucleic acids research · 2022Article
- A Proximity biotinylation assay with a host protein bait reveals multiple factors modulating enterovirus replication.PLoS pathogens · 2022Article
- CTCF and cohesin promote focal detachment of DNA from the nuclear lamina.Genome biology · 2022Article
- CTCF supports preferentially short lamina-associated domains.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Article
- Advances in enzyme-mediated proximity labeling and its potential for plant research.Plant physiology · 2022Article
- Proximity-dependent biotinylation technologies for mapping RNA-protein interactions in live cells.Frontiers in molecular biosciences · 2022Review
- Measuring Cytological Proximity of Chromosomal Loci to Defined Nuclear Compartments with TSA-seq.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Lamin post-translational modifications: emerging toggles of nuclear organization and function.Trends in biochemical sciences · 2021Review
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Authors and funding
7 authors.
Funding
Abstract
The nuclear lamina (NL) is a meshwork found beneath the inner nuclear membrane. The study of the NL is hindered by the insolubility of the meshwork and has driven the development of proximity ligation methods to identify the NL-associated/proximal proteins, RNA, and DNA. To simplify and improve temporal labeling, we fused APEX2 to the NL protein lamin-B1 to map proteins, RNA, and DNA. The identified NL-interacting/proximal RNAs show a long 3' UTR bias, a finding consistent with an observed bias toward longer 3' UTRs in genes deregulated in lamin-null cells. A C-rich motif was identified in these 3' UTR. Our APEX2-based proteomics identifies a C-rich motif binding regulatory protein that exhibits altered localization in lamin-null cells. Finally, we use APEX2 to map lamina-associated domains (LADs) during the cell cycle and uncover short, H3K27me3-rich variable LADs. Thus, the APEX2-based tools presented here permit identification of proteomes, transcriptomes, and genome elements associated with or proximal to the NL.
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