Evidence map›Paper›PMID 33305885›Full record

ArticleInsect molecular biology2021

Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest.

K B Ferguson, S Visser, M Dalíková, I Provazníková, A Urbaneja, M Pérez-Hedo, F Marec, J H Werren, B J Zwaan, B A Pannebakker and 1 more

Open access · hybridAbstract read
In one paragraph

Article in Insect molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 28 citations in OpenAlex.

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  14. Analysis of Holhymenia histrio genome provides insight into the satDNA evolution in an insect with holocentric chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 5 countries.

K B FergusonLaboratory of Genetics, Wageningen University, Wageningen, The Netherlands.ORCID 0000-0001-6483-1936
S VisserBiology Centre CAS, Institute of Entomology, České Budějovice, Czech Republic.
M DalíkováBiology Centre CAS, Institute of Entomology, České Budějovice, Czech Republic.ORCID 0000-0002-0266-8894
I ProvazníkováBiology Centre CAS, Institute of Entomology, České Budějovice, Czech Republic.ORCID 0000-0003-2336-458X
A UrbanejaCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Spain.ORCID 0000-0001-5986-3685
M Pérez-HedoCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Spain.ORCID 0000-0003-3411-0761
F MarecBiology Centre CAS, Institute of Entomology, České Budějovice, Czech Republic.ORCID 0000-0002-6745-5603
J H WerrenDepartment of Biology, University of Rochester, Rochester, New York, USA.ORCID 0000-0001-9353-2070
B J ZwaanLaboratory of Genetics, Wageningen University, Wageningen, The Netherlands.ORCID 0000-0002-8221-4998
B A PannebakkerLaboratory of Genetics, Wageningen University, Wageningen, The Netherlands.ORCID 0000-0001-8503-3896
E C VerhulstLaboratory of Entomology, Wageningen University, Wageningen, The Netherlands.ORCID 0000-0002-4781-535X
Wageningen University & Research · NLUniversity of South Bohemia in České Budějovice · CZInstituto Valenciano de Investigaciones Agrarias · ESInstitute of Entomology · CZUniversity of Rochester · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout the Mediterranean Basin in tomato crops but regarded as a pest in northern European countries. From the family Miridae, it is an economically important insect yet very little is known in terms of genetic information and no genomic or transcriptomic studies have been published. Here, we use a linked-read sequencing strategy on a single female N. tenuis. From this, we assembled the 355 Mbp genome and delivered an ab initio, homology-based and evidence-based annotation. Along the way, the bacterial "contamination" was removed from the assembly. In addition, bacterial lateral gene transfer (LGT) candidates were detected in the N. tenuis genome. The complete gene set is composed of 24 688 genes; the associated proteins were compared to other hemipterans (Cimex lectularis, Halyomorpha halys and Acyrthosiphon pisum). We visualized the genome using various cytogenetic techniques, such as karyotyping, CGH and GISH, indicating a karyotype of 2n = 32. Additional analyses include the localization of 18S rDNA and unique satellite probes as well as pooled sequencing to assess nucleotide diversity and neutrality of the commercial population. This is one of the first mirid genomes to be released and the first of a mirid biological control agent.

Indexed as

AnimalsBacteriaBiological Control AgentsFemaleGene Transfer, HorizontalGenome, InsectHeteropteraSymbiosisBiological Control AgentsbiocontrolHemipteralinked-read

Identifiers

PMID33305885
PMCPMC8048687
OpenAlexW3111874862

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.