ArticleFrontiers in microbiology2020
Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Yeast culture promotes butyrate produced fibrolytic bacteria as intracellular hydrogen sink in the rumen.Microbiome · 2026Article
- Construction of Synthetic Microbial Community with Core Microorganisms for Soy Sauce Fermentation.Foods (Basel, Switzerland) · 2026Article
- Effects of Yeast Cultures on Growth Performance, Fiber Digestibility, Ruminal Dissolved Gases, Antioxidant Capacity and Immune Activity of Beef Cattle.Animals : an open access journal from MDPI · 2025Article
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- Influence of Dietary Forage Neutral Detergent Fiber on Ruminal Fermentation, Chewing Activity, Nutrient Digestion, and Ruminal Microbiota of Hu Sheep.Animals : an open access journal from MDPI · 2025Article
- Metabolic versatility enables acetogens to colonize ruminants with diet-driven niche partitioning.The ISME journal · 2025Article
- Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community.Animals : an open access journal from MDPI · 2024Article
- Aloe arborescens supplementation in drying-off dairy cows: influence on rumen, rectum and milk microbiomes.Animal microbiome · 2024Article
- Detection ofMicroorganisms · 2024Article
- Effects of dietary forage neutral detergent fiber and rumen degradable starch ratios on chewing activity, ruminal fermentation, ruminal microbes and nutrient digestibility of Hu sheep fed a pelleted total mixed ration.Journal of animal science · 2024Article
- Exploring the gut microbiota in patients with pre-diabetes and treatment naïve diabetes type 2 - a pilot study.BMC endocrine disorders · 2023Article
- Evaluation of the representative of using rumen fluid samples from lambs fed pelleted TMR for analysis of prokaryotic communities.Frontiers in microbiology · 2023Article
- Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities.Frontiers in microbiology · 2023Article
- Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.The ISME journal · 2022Article
- Article
- Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies.Journal of animal science · 2022Review
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- Comparison ofFrontiers in microbiology · 2022Article
- Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits.Applications in plant sciences · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
QIAamp Fast DNA Stool Mini Kit (QIAGEN, Valencia, CA, United States) and RBB + C (Yu and Morrison, 2004) methodologies are widely employed to extract microbial DNA from rumen samples and can exhibit different efficiencies of obtaining DNA yield, quality, and downstream amplicon sequence analysis. No study has conducted to investigate the contributions of chemical and mechanical lysis on DNA extraction, which included chemical lysis from QIAamp Fast DNA Stool Mini Kit (QIA) and RBB + C (YM), bead (BB), and sand beating (SB). Effects of chemical lysis and bead beating (BB) were investigated by conducting a 2 × 2 factorial-designed experiment with four methodologies, including QIA without (QIA-) and with BB (QIA + BB), and YM without (YM-) and with BB (YM + BB). Comparisons between bead and sand were conducted by comparing methodologies of YM + BB and YM + SB. Comparing with QIA, YM had lower (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.