Evidence map›Paper›PMID 33304198›Full record

ArticleProcess biochemistry (Barking, London, England)2021

Isolation and detection of a KDEL-tagged recombinant cholera toxin B subunit from

David A Morris, Micaela A Reeves, Joshua M Royal, Krystal T Hamorsky, Nobuyuki Matoba

Abstract read
In one paragraph

Article in Process biochemistry (Barking, London, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Plant-based edible vaccines: Can cholera be the case study in Africa?Journal, genetic engineering & biotechnology · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

David A MorrisJames Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.
Micaela A ReevesDepartment of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY, USA.
Joshua M RoyalJames Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.
Krystal T HamorskyJames Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.
Nobuyuki MatobaJames Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.

Funding

Preclinical validation of oral therapeutic lead proteins targeting epithelial GM1 ganglioside for ulcerative colitis therapyR01DK123712 · NIDDK · UNIVERSITY OF LOUISVILLE · PI MATOBA, NOBUYUKI · 2020 to 2023
$1.8M
NIDDK NIH HHS R01 DK123712
6 · The paper itself

Abstract

Here we describe refined methods for the isolation and detection of a KDEL-tagged, plant-produced recombinant cholera toxin B subunit (CTB) that exhibits unique mucosal wound healing activity. The protein was transiently overexpressed in

Indexed as

biotherapeuticceramic hydroxyapatite chromatographyC-terminal truncationER retentionimmunoassayplant-made pharmaceutical

Identifiers

PMID33304198
PMCPMC7723343

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.