ArticleSaudi journal of biological sciences2020
Immune effects of miRNA and Th17 cells on β-Lg allergy in dietary milk based on mouse model.
Article in Saudi journal of biological sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.
- MicroRNA expression and their molecular targets in food allergies: a systematic review.Frontiers in immunology · 2025Pooled it
- [Effect of interleukinZhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2025Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
β-lactoglobulin (β-Lg) allergy in dietary milk seriously affects the use of high-quality milk protein in infants. In order to solve this problem, the expression of miRNA and Th17 cells in milk β-Lg allergic reaction of children's diet was studied.
methodfemale BALB/c mice aged 5-6 weeks were selected as the subjects and randomly divided into blank group and β-Lg sensitized group, with 10 mice in each group. On the 1st, 7th and 14th day, the mice in the β-Lg sensitized group were intraperitoneally injected with allergen (Freund's adjuvant + β-Lg). Mice in the blank group were given the same amount of normal saline. Blood samples were collected from the eyeballs of mice to determine the number of inflammatory cells. The contents of Th17 related cytokines and transcription factors in spleen were detected by RT-PCR.
results1. the number of eosinophils and neutrophils in the β-Lg sensitized group were 15.76/mL and 24.36/mL, respectively, which were significantly higher than those in the blank group (P < 0.05); 2. in the mice of β-Lg sensitized group, the expression of miR-146a and miR-155 was abnormal, the number of Th17 cells was abnormally increased, and the expression levels of IL-17 and RORγt were significantly increased; 3. the abnormal expression of miR-146a and miR-155 in the mice of β-Lg sensitized group was positively correlated with the secretion of Th17 related cytokines, which could be used as one of the biological indexes to evaluate allergic reaction.
conclusionthe number of Thl7 increased abnormally in dietary milk allergy patients. miRNA gene expression and IL-17 expression could be used as one of the biological indicators to evaluate the allergic reaction of β-Lg.
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