ArticleScientific reports2020
MRI-based molecular imaging of epicardium-derived stromal cells (EpiSC) by peptide-mediated active targeting.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Impact of fluorine-containing nanoparticle PEGylation on inflammation imaging byScientific reports · 2025Article
- Imaging of Thromboinflammation by MultispectralInternational journal of molecular sciences · 2025Review
- CD63 as novel target for nanoemulsion-basedTheranostics · 2025Article
- A Toolbox to Investigate the Impact of Impaired Oxygen Delivery in Experimental Disease Models.Frontiers in medicine · 2022Review
- Epicardial Contribution to the Developing and Injured Heart: Exploring the Cellular Composition of the Epicardium.Frontiers in cardiovascular medicine · 2021Review
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After myocardial infarction (MI), epicardial cells reactivate their embryonic program, proliferate and migrate into the damaged tissue to differentiate into fibroblasts, endothelial cells and, if adequately stimulated, to cardiomyocytes. Targeting epicardium-derived stromal cells (EpiSC) by specific ligands might enable the direct imaging of EpiSCs after MI to better understand their biology, but also may permit the cell-specific delivery of small molecules to improve the post-MI healing process. Therefore, the aim of this study was to identify specific peptides by phage display screening to enable EpiSC specific cargo delivery by active targeting. To this end, we utilized a sequential panning of a phage library on cultured rat EpiSCs and then subtracted phage that nonspecifically bound blood immune cells. EpiSC specific phage were analyzed by deep sequencing and bioinformatics analysis to identify a total of 78 300 ± 31 900 different, EpiSC-specific, peptide insertion sequences. Flow cytometry of the five most highly abundant peptides (EP1, -2, -3, -7 or EP9) showed strong binding to EpiSCs but not to blood immune cells. The best binding properties were found for EP9 which was further studied by surface plasmon resonance (SPR). SPR revealed rapid and stable association of EpiSCs with EP9. As a negative control, THP-1 monocytes did not associate with EP9. Coupling of EP9 to perfluorocarbon nanoemulsions (PFCs) resulted in the efficient delivery of
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