ArticleMicrobial cell factories2020
Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- In vivo systemic toxicity assessment of Bacillus safensis NMCC-189: a probiotic strain evaluated via short-term tolerability and 28-day repeated-dose (OECD 407) study in Balb/c mice.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Mechanistic and applied study of phosphofructokinases, the "gatekeeper" of the glycolytic pathway on the central carbon metabolism.Metabolic engineering communications · 2026Article
- Construction of engineered Streptomyces albulus by increasing ATP and NADH supply and efficient production of ε-poly-L-lysine from sweet potato powder.World journal of microbiology & biotechnology · 2026Article
- Regulating Glycerol Metabolism to Investigate the Effects of EngineeredFoods (Basel, Switzerland) · 2026Article
- The advances in creating Crabtree-negative Saccharomyces cerevisiae and the application for chemicals biosynthesis.FEMS yeast research · 2025Review
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- A comparative analysis of NADPH supply strategies inMetabolic engineering communications · 2024Article
- Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel.AMB Express · 2024Article
- Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production.Biotechnology for biofuels and bioproducts · 2023Article
- Key enzymes involved in the utilization of fatty acids byFrontiers in microbiology · 2023Review
- Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects inSynthetic and systems biotechnology · 2022Article
- Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and β-alanine metabolism.Microbial biotechnology · 2022Article
- Multiplex Genome Editing in Yeast by CRISPR/Cas9 - A Potent and Agile Tool to Reconstruct Complex Metabolic Pathways.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundSaccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex expressed in the yeast cytosol was reported to enable production of cytosolic acetyl-CoA with lower energy cost and no toxic intermediate.
resultsOverexpression of the PDH complex significantly increased cell growth, ethanol consumption and reduced glycerol accumulation. Furthermore, to optimize the redox imbalance in production of fatty acids from glucose, two endogenous NAD
conclusionsWe demonstrated that cytosolic PDH pathway enabled more efficient acetyl-CoA provision with the lower ATP cost, and improved FFA production. Together with engineering of the redox factor rebalance, the cytosolic PDH pathway could achieve high level of FFA production at similar levels of other best acetyl-CoA producing pathways.
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