ArticleMethods in molecular biology (Clifton, N.J.)2021
The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
108 citing papers in PubMed.
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence of kidney vetch,Wellcome open research · 2026Article
- The genome sequence of the daisy,Wellcome open research · 2026Article
- The genome sequence of Bastard Balm,Wellcome open research · 2026Article
- The genome sequence of Slender Tare,Wellcome open research · 2026Article
- The genome sequence of dewberry,Wellcome open research · 2026Article
- The genome sequence of flixweed,Wellcome open research · 2026Article
- The genome sequence of the sweet violet,Wellcome open research · 2026Article
- The genome sequence of the starved wood-sedge,Wellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
- Insights into dill (Anethum graveolens) flavor formation via integrative analysis of chromosomal-scale genome, metabolome and transcriptome.Journal of advanced research · 2026Article
- The genome sequence ofWellcome open research · 2026Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the years, the amount of DNA in a nucleus (genome size) has been estimated using a variety of methods, but increasingly, flow cytometry (FCM) has become the method of choice. The popularity of this technique lies in the ease of sample preparation and in the large number of particles (i.e., nuclei) that can be analyzed in a very short period of time. This chapter presents a step-by-step guide to estimating the nuclear DNA content of plant nuclei using FCM. Attempting to serve as a tool for daily laboratory practice, we list, in detail, the equipment required, specific reagents and buffers needed, as well as the most frequently used protocols to carry out nuclei isolation. In addition, solutions to the most common problems that users may encounter when working with plant material and troubleshooting advice are provided. Finally, information about the correct terminology to use and the importance of obtaining chromosome counts to avoid cytological misinterpretations of the FCM data are discussed.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.