ArticleMethods in molecular biology (Clifton, N.J.)2021
Inter-Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Integrated morphological, pomological, biochemical, and ISSR-based assessment of genetic diversity in wild sour orange (Citrus aurantium L.) genotypes from the Hassa district of Hatay, Türkiye.Biological research · 2026Article
- Phenotyping elucidates the association between traits and bioactive components in Notopterygium incisum.BMC plant biology · 2026Article
- Article
- Factors affecting the genetic diversity ofFrontiers in plant science · 2026Review
- Genetic diversity analysis ofFrontiers in plant science · 2025Article
- Genetic Mapping and Characterization of the Clubroot Resistance GeneInternational journal of molecular sciences · 2024Article
- Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free 'Shine Muscat' Grapevine Cultivar.Current issues in molecular biology · 2023Article
- Efficient shoot regeneration of medicinal plantFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inter-simple sequence repeat (ISSR) markers are highly polymorphic, relatively easy to develop, and inexpensive compared to other methods and have numerous applications. Importantly, the same ISSR primers can potentially be used universally across plant phylogenetic diversity. The basic technique of ISSRs is flexible and can be modified with options for implementation for a broad range of projects and budgets. Ranked in increasing order of technical demand and costs, these are manual agarose and manual polyacrylamide with silver staining and automated using fluorescently labeled primers and capillary electrophoresis. Overall manual agarose-based ISSRs are a sound, safe, easy, and low-cost method for reliably inferring plant genetic diversity. Here, we provide detailed protocols to undertake this fingerprinting method and provide guidance to the literature for the many options available for this technique.
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Registered trials
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