Evidence map›Paper›PMID 33301096›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2021

Amplified Fragment Length Polymorphism: Applications and Recent Developments.

Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, Kantipudi Nirmal Babu

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
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  3. Review
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  7. Article
  8. What Can Genetics Do for the Control of Infectious Diseases in Aquaculture?Animals : an open access journal from MDPI · 2022
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Thotten Elampilay SheejaIndian Institute of Spices Research, Kozhikode, Kerala, India. tesheeja@gmail.com.
Illathidath Payatatti Vijesh KumarIndian Institute of Spices Research, Kozhikode, Kerala, India.
Ananduchandra GiridhariIndian Institute of Spices Research, Kozhikode, Kerala, India.
Divakaran MinooProvidence Women's College, Kozhikode, Kerala, India.
Muliyar Krishna RajeshCentral Plantation Crops Research Institute, Kasaragod, Kerala, India.
Kantipudi Nirmal BabuIndian Institute of Spices Research, Kozhikode, Kerala, India.
Indian Institute of Spices Research · INCentral Plantation Crops Research Institute · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes. It is more efficient in terms of time, economy, reproducibility, informativeness, resolution, and sensitivity, compared to other popular DNA markers. Besides, it requires very small quantities of DNA and no prior genome information. This technique is widely used in plants for taxonomy, genetic diversity, phylogenetic analysis, construction of high-resolution genetic maps, and positional cloning of genes, to determine relatedness among cultivars and varietal identity, etc. The review encompasses in detail the various applications of AFLP in plants and the major advantages and disadvantages. The review also considers various modifications of this technique and novel developments in detection of polymorphism. A wet-lab protocol is also provided.

Indexed as

Amplified Fragment Length Polymorphism AnalysisDNA Barcoding, TaxonomicDNA FingerprintingEpigenomicsGene Expression ProfilingGene Expression Regulation, PlantGenetic VariationPlantsTranscriptomeAFLPcDNAEpigeneticsGenetic diversityMSAPRestriction enzymesTranscriptomics

Identifiers

PMID33301096
OpenAlexW3112142939

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.