Evidence map›Paper›PMID 33299805›Full record

ArticleMethodsX2020

Protocol for efficient fluorescence 3' end-labeling of native noncoding RNA domains.

Dahlia A Awwad, A Rachid Rahmouni, Fareed Aboul-Ela

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. ATP:MgBiophysical journal · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dahlia A AwwadCenter of X-Ray Determination of Structure of Matter (CXDS), Helmy Institute of Medical Sciences, Zewail City of Science and Technology, Giza, Egypt.
A Rachid RahmouniCentre de Biophysique Moléculaire, UPR4301 du CNRS, Orléans, France.
Fareed Aboul-ElaCenter of X-Ray Determination of Structure of Matter (CXDS), Helmy Institute of Medical Sciences, Zewail City of Science and Technology, Giza, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Noncoding RNAs (ncRNAs) comprise a class of versatile transcripts that are highly involved in the regulation of a wide range of biological processes. Functional long ncRNAs (> 200 nts in length) often adopt secondary structures that arise co-transcriptionally. To maintain the secondary structure elements as well as preparation homogeneity of such transcripts, native-like conditions should be maintained throughout the

Indexed as

3′end-labelingChemical taggingCyanine hydrazidesFluorescencein vitro transcriptionNative purificationRNA

Identifiers

PMID33299805
PMCPMC7704408

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.