Evidence map›Paper›PMID 33299140›Full record

ArticleCancer gene therapy2021

microRNA-let-7e in serum-derived exosomes inhibits the metastasis of non-small-cell lung cancer in a SUV39H2/LSD1/CDH1-dependent manner.

Shufeng Xu, Lei Zheng, Liying Kang, Hongmei Xu, Liming Gao

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Cancer gene therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Shufeng XuDepartment of Respiratory, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, PR China.
Lei ZhengDepartment of Oncology, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, PR China.
Liying KangDepartment of Oncology, Tianjin Wuqing District People's Hospital, Tianjin, 301700, PR China.
Hongmei XuDepartment of Oncology, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, PR China.
Liming GaoDepartment of Oncology, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, PR China. Tshgaoliming@sina.com.ORCID http://orcid.org/0000-0003-3284-9896
First Hospital of Qinhuangdao · CNWuqing District People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer-related death worldwide. Accumulating research has highlighted the ability of exosome-encapsulated microRNAs (miRNAs or miRs) as potential circulating biomarkers for lung cancer. The current study aimed to evaluate the clinical significance of serum-derived exosomal miR-let-7e as a biomarker in the metastasis of NSCLC. Initially, the expression of miR-let-7e, SUV39H2, and CDH1 in human NSCLC tissues and exosomes isolated from the serum of NSCLC patients was determined by RT-qPCR, demonstrating that miR-let-7e was downregulated in NSCLC tissues and serum-derived exosomes, while SUV39H2 was upregulated in NSCLC tissues. Kaplan-Meier method revealed that both lower miR-let-7e expression and higher SUV39H2 expression were correlated with a lower survival rate of NSCLC patients. Next, SUV39H2 was predicted and validated to be a target of miR-let-7e using dual-luciferase reporter assay. NSCLC H1299 cells following ectopic expression and depletion experiments of miR-let-7e and SUV39H2 were treated with serum-derived exosomes, after which the viability, migration, and invasion of H1299 cells were detected using CCK-8 and Transwell assays. Further, in vivo experiments were conducted to elucidate the effect of exosomal miR-let-7e on tumorigenesis. Results revealed that miR-let-7e overexpression in serum-derived exosomes inhibited SUV39H2, resulting in impaired cell viability, migration, and invasion in vitro as well as delayed tumor growth in vivo. In conclusion, the key findings of the current study demonstrate that exosomal miR-let-7e from serum possesses anticarcinogenic properties against NSCLC via the SUV39H2/LSD1/CDH1 axis.

Indexed as

AnimalsAntigens, CDCadherinsCarcinoma, Non-Small-Cell LungCase-Control StudiesDisease Models, AnimalExosomesHeterograftsHistone DemethylasesHistone-Lysine N-MethyltransferaseHumansLung NeoplasmsMaleMicroRNAsNeoplasm MetastasisTransfectionAntigens, CDCadherinsCDH1 protein, humanHistone DemethylasesHistone-Lysine N-MethyltransferaseKDM1A protein, humanMicroRNAsmirnlet7 microRNA, humanSUV39H2 protein, human

Identifiers

PMID33299140
OpenAlexW3112195253

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.