ArticleScientific reports2020
Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- BMP-7 Enhances the Angiogenic Potential of Wharton's Jelly Mesenchymal Stem Cells Seeded in Decellularized Human Dental Pulp.International journal of molecular sciences · 2026Article
- Therapeutic potential of curcumin in regenerative dentistry.Frontiers in dental medicine · 2025Article
- Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study.BMC oral health · 2024Article
- Bovine pulp extracellular matrix hydrogel for regenerative endodontic applications: in vitro characterization and in vivo analysis in a necrotic tooth model.Head & face medicine · 2024Article
- Creating a Microenvironment to Give Wings to Dental Pulp Regeneration-Bioactive Scaffolds.Pharmaceutics · 2023Review
- The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review.Materials (Basel, Switzerland) · 2022Review
- Potential of extracellular space for tissue regeneration in dentistry.Frontiers in physiology · 2022Review
- Clinical Potential of Dental Pulp Stem Cells in Pulp Regeneration: Current Endodontic Progress and Future Perspectives.Frontiers in cell and developmental biology · 2022Review
- Functional Dental Pulp Regeneration: Basic Research and Clinical Translation.International journal of molecular sciences · 2021Review
- Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications.Polymers · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Implementing the principles of tissue engineering within the clinical management of non-vital immature permanent teeth is of clinical interest. However, the ideal scaffold remains elusive. The aim of this work was to assess the feasibility of decellularising rat dental pulp tissue and evaluate the ability of such scaffold to support stem cell repopulation. Rat dental pulps were retrieved and divided into control and decellularised groups. The decellularisation protocol incorporated a low detergent concentration and hypotonic buffers. After decellularisation, the scaffolds were characterised histologically, immunohistochemistry and the residual DNA content quantified. Surface topography was also viewed under scanning electron microscopy. Biocompatibility was evaluated using cytotoxicity assays utilising L-929 cell line. Decellularised scaffolds were recellularised with human dental pulp stem cells up to 14 days in vitro. Cellular viability was assessed using LIVE/DEAD stain kit and the recellularised scaffolds were further assessed histologically and immunolabelled using makers for odontoblastic differentiation, cytoskeleton components and growth factors. Analysis of the decellularised scaffolds revealed an acellular matrix with histological preservation of structural components. Decellularised scaffolds were biocompatible and able to support stem cell survival following recellularisation. Immunolabelling of the recellularised scaffolds demonstrated positive cellular expression against the tested markers in culture. This study has demonstrated the feasibility of developing a biocompatible decellularised dental pulp scaffold, which is able to support dental pulp stem cell repopulation. Clinically, decellularised pulp tissue could possibly be a suitable scaffold for use within regenerative (reparative) endodontic techniques.
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