ArticleScientific reports2020
Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 71 citations in OpenAlex.
- IMD-0354 optimization generates potent colistin adjuvants with in vivo activity and reduced eukaryotic toxicity.European journal of medicinal chemistry · 2026Article
- Combinatorial action of regulatory systems generates colistin heteroresistance.Science advances · 2026Article
- Phosphoethanolamine modification of lipid A found in five distinctMicrobiology spectrum · 2026Article
- Phosphoethanolamine-modified lipid A enables antimicrobial resistance and atypical signaling properties in the marine pathogenMicrobiology spectrum · 2026Article
- Genomic, proteolytic, and phenotypic characterization ofMicrobiology spectrum · 2026Article
- A Culture-Free Lipidomics-Based Screening Test for Uropathogens.Clinical chemistry · 2026Article
- DMEM and EMEM as alternate growth media for pathogenic Leptospira.PLoS neglected tropical diseases · 2026Article
- A Pilot Study Using Microbial Glycolipid Signatures to Diagnose Nosocomial Ventriculitis.Rapid communications in mass spectrometry : RCM · 2025Article
- A critical role forProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Current Methods for Reliable Identification of Species in theMicroorganisms · 2025Review
- Rapid Multi-Omics for Bacterial Identification Using Flow Injection-Ion Mobility-Mass Spectrometry.Analytical chemistry · 2025Article
- Rapid MALDI-MS/MS-Based Profiling of Lipid A Species from Gram-Negative Bacteria Utilizing Trapped Ion Mobility Spectrometry and mzmine.Analytical chemistry · 2025Article
- BECC-engineered live-attenuated Shigella vaccine candidates display reduced endotoxicity with robust immunogenicity in mice.Vaccine · 2025Article
- DMEM and EMEM are suitable surrogate media to mimic host environment and expand leptospiral pathogenesis studies usingbioRxiv : the preprint server for biology · 2025Article
- Review
- Site-selective modifications by lipid A phosphoethanolamine transferases linked to colistin resistance and bacterial fitness.mSphere · 2024Article
- A lipidomics-based method to eliminate negative urine culture in general population.Journal of clinical microbiology · 2024Article
- Evaluating the accuracy of the MBT Lipid Xtract Kit for assessing colistin resistance in comparison to broth microdilution.Journal of medical microbiology · 2024Article
- A Multimodal System for Lipid A Structural Analysis from a Single Colony.Analytical chemistry · 2024Article
- Intact cell lipidomics using the Bruker MBT lipid Xtract assay allows the rapid detection of glycosyl-inositol-phospho-ceramides fromMolecular omics · 2024Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.