ArticleScientific reports2020
Field application of an improved protocol for environmental DNA extraction, purification, and measurement using Sterivex filter.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Environmental DNA sampling workflow matters: testing different approaches on marine microbial assemblages in three coastal areas of Greece.Frontiers in microbiology · 2026Article
- A comparison of microbial community composition in two alpine springs in southern Nevada.PloS one · 2026Article
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- Shifting seas and first feeds: gut microbiome dynamics in juvenile chum salmon (Current research in microbial sciences · 2025Article
- Article
- Improving the efficiency of adaptive management methods in multiple fishways using environmental DNA.PloS one · 2024Article
- Benchmarking DNA isolation methods for marine metagenomics.Scientific reports · 2023Article
- Methodological considerations for aqueous environmental RNA collection, preservation, and extraction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Improvement of recovery yield of macro-organismal environmental DNA from seawater samples.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean.Ecology and evolution · 2023Article
- What samples are suitable for monitoring antimicrobial-resistant genes? Using NGS technology, a comparison between eDNA and mrDNA analysis from environmental water.Frontiers in microbiology · 2023Article
- Article
- Long Term Copepod Culture Houses a Rich Microbial Eukaryotic Community Including New and Known Symbionts.The Journal of eukaryotic microbiologyArticle
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3 authors.
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Abstract
Environmental DNA (eDNA) is increasingly popular as a useful non-invasive method to monitor and study biodiversity and community structure in freshwater and marine environments. To effectively extract eDNA from the filter surface is a fundamental factor determining the representativeness of the samples. We improved the eDNA extraction efficiency of an established Sterivex method by 12- to 16-fold using a larger volume of lysis buffer mix coupled with backflushing the cartridges. The DNeasy extraction column could be overloaded when the environmental sample input is high, possibly due to a higher nonspecific binding present in environmental samples, thus resulting in a relatively lower quantity measured. Therefore, we included an internal control DNA in the extraction to monitor the extraction and purification efficiencies in field samples, which is crucial for quantification of original eDNA concentration. The use of Takara Probe qPCR Mix supplemented with protein-based additives improved the robustness of the real time PCR assay on inhibitor-rich environmental samples, but prior purification by Qiagen PowerClean Pro Cleanup kit could be essential for inhibitor-rich water samples, even though the recovery rate was unexpectedly low (average 33.0%). The improved extraction and quantification complement the qualitative analyses including metabarcoding and metagenomics in field application.
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